Since the early morning of the 29th, Zhengzhou has "boiled", and we have once again won the battle against the new crown epidemic. Since the detection of local cases in Zhengzhou on January 3, the medium-risk areas designated for epidemic prevention and control in Zhengzhou have all been reduced to low-risk areas! We successfully "picked the stars".
"The years are quiet, and because of the countless star pickers, they are carrying the weight forward." At the time of picking the stars, we still deeply realize that "low risk does not equal zero risk", and always be prepared for war, in order to never lose a battle.
So, what should we do in the gap between the epidemic prevention and control war? As a team that has been involved in nucleic acid screening many times, we feel that it is extremely important to complete the following work preparation!
Personnel training tamping down the level
Strengthening personnel training is something that nucleic acid testing teams often do tirelessly. First of all, the internal personnel of the team should be trained, the experience should be exchanged and shared, and the abnormal amplification analysis should be continuously improved to continuously improve the ability and experience of accurate reporting.
In addition, during the epidemic period, the routine work of the laboratory department will be greatly reduced, and the amount of nucleic acid screening will increase sharply, and sometimes more than half of the personnel of the department need to be mobilized to participate in nucleic acid testing, so it is urgent to strengthen the training of new personnel and do a good job in personnel reserves.
Maintenance equipment Follow-up maintenance
Nucleic acid extraction instrument and nucleic acid amplification instrument are the main equipment for nucleic acid screening and detection. During the epidemic prevention and control period, in order to complete the testing task in a timely manner, all the instruments and equipment operated for almost 24 hours. If it cannot be maintained and maintained in time, once it fails in the application process, it will seriously affect the efficiency of nucleic acid detection.
If the fault is obvious, it only needs to be newly formulated from the reaction system, and the equipment can be replaced and amplified to make up for it. Some faults that are not easy to find often lead to abnormal amplification curves, which seriously interfere with the judgment of the results. Based on this, in the gap between the epidemic, it is extremely important to carry out comprehensive overhaul, verification, maintenance and maintenance of equipment.
Performance verification Optimized preferred
At present, there are many types of reagents used in new crown nucleic acid detection, and each reagent has its own advantages and disadvantages in terms of sensitivity and specificity. According to the requirements, when there are suspicious results in the laboratory, it is necessary to use different methods and principles of reagents to repeatedly verify before reporting, so we should verify the performance of various reagents before the reagent is used to understand its advantages and disadvantages in detail, and truly know what is in mind.
In addition, different kinds of reagents, the nucleic acid extraction time and the length of on-the-machine amplification time are different, in order to minimize TAT and provide valuable time for epidemic prevention and control, we can effectively combine different reagents under the premise of allowing to obtain the required TAT or result accuracy.
Process optimization Scientific and reasonable
The nucleic acid detection process involves multiple links, and any defect in any link will cause the testing process to be unsmooth, the result reporting will be delayed, and even affect the accuracy of the results. Therefore, when the batch detection is reduced, the detection process can be further optimized according to the gains and losses in operation.
If the specimen transfer box is placed reasonably, avoiding the mixed placement of the transfer box with /without specimens, causing specimen omission; the registration is clear and clear when the specimen is received, not only the number of mixed pipes, it is best to have the screening number of people checking the record; the information entry is timely and accurate, to avoid the delay in receiving the results of the screening examiner; the nucleic acid extraction, nucleic acid dosing and amplification personnel are arranged to coordinate reasonably, ensure that the process is orderly, and shorten the testing time; do a good job of re-examination rules and re-inspection plans to ensure that the re-examination of suspicious/positive specimens is timely; the audit specifications are timely and accurate to avoid missed and mis-examination.
Summarize and summarize to improve the results of the battle
Nucleic acid testing is the main basis for the screening, diagnosis, efficacy assessment, prognosis judgment, and the formulation of measures for isolation and unsealing of epidemic areas. However, simply achieving this use somewhat weakens the value of clinical testing.
In fact, from the laboratory point of view, the evaluation of methods related to the diagnosis of new crown pneumonia, the formulation of quality control measures, the performance verification of reagents and the verification of the equipment used will become the content of clinical laboratory research; from the perspective of experimental diagnosis, it is more important to explore the compatibility of test results with the progress of the patient's condition, gradually improve the degree of accurate diagnosis and precise treatment; from the perspective of scientific research, successfully establish a new method, screen a new index, or evaluate a new clinical application. Both are expected to become remarkable business achievements.
In short, only by truly "unsealing and not solving the defense, and stopping the war and not hiding the knife" can we win the epidemic prevention and control war again and again, and can we truly realize the original intention of the doctor.
Source: Dean Experimental Diagnosis
Edited by: Yeah Reviewer: Xiao Ran