L-leucine is one of the eight essential amino acids of the human body, belonging to the aliphatic amino acids in twenty protein amino acids, isoleucine is an isomer of leucine, the isobutyl group of the leucine side chain is replaced by a sec-butyl group, leucine, isoleucine and L-valine are three branched-chain amino acids. It can prepare indispensable components such as amino acid infusion, comprehensive amino acid preparations, and hypoglycemic agents, and is widely used in biomedicine, food industry and other fields. Accurate and efficient detection of L-isoleucine during production fermentation is critical.
The main methods for detecting isoleucine content include high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and automatic amino acid analyzer. Capillary electrophoresis (CE) uses an electric field to migrate charged amino acids in a capillary to separate and detect them according to the migration time, while an automatic amino acid analyzer uses a specific separation column and detector to automatically separate and detect amino acids.
Based on the detection methods of amino acids in the literature, the microsource testing laboratory established a reversed-phase high-performance liquid chromochromatic to determine several amino acids such as leucine and isoleucine. The resulting method showed that the resolution met the requirements. Sample spike recovery was determined. The method is highly accurate, reliable, quick and easy to operate, and can be injected directly. It can be used for quality control in the food industry and the pharmaceutical industry.
Amino acids are the basic building blocks of biofunctional macromolecular proteins, and they are the basic substances that constitute the proteins required for animal nutrition. The detection of amino acid content is difficult to study the structure, function and metabolic pathways of proteins, but it is difficult to separate and detect most amino acids due to their high polarity, low volatility and lack of strong luminescent groups. Which method to choose depends on the conditions of the laboratory, the availability of the instrument, and the type of sample. In general, HPLC is widely used for the detection of amino acids due to its high sensitivity, accuracy, and ease of operation.
The microsource testing laboratory uses high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) to provide detection services for amino acids and their metabolites such as isoleucine, Ile, etc. As a testing platform of a listed company, it uses more than 100 instruments and equipment such as spectrum, chromatography, mass spectrometry, energy spectroscopy, and thermal spectroscopy to comprehensively analyze samples, and provides professional testing services for enterprises and institutions in biological, pharmaceutical, chemical and other fields.
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