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Moderator: Hello everyone, this is the "Rural Micro Classroom" of Shanxi Rural Science and Technology Correspondence University, today we are very fortunate to invite Teacher Wang Hua of the Institute of Edible Mushrooms of Shanxi Academy of Agricultural Sciences. Hello, Teacher Wang.
Teacher Wang: Hello host, hello everyone.
Host: So in the last episode we talked about the bagging of bailing mushrooms, so after bagging, which step should we take?
Teacher Wang: After bagging, we will sterilize and inoculate, and after packing the bag, we can sterilize in two ways, one is atmospheric pressure, and the other is high pressure. When the pressure is high pressure, how much to keep under the pressure of 1.47 multiplied by 155 pascals, if you are atmospheric pressure sterilization, you must extinguish 10 to 12 hours, and then naturally cool after in the sterile room or in the inoculation box, and then access the strain of this white spirit mushroom, practice has proved that it is generally 500 ml of wheat seeds, it can be inoculated with 20 to 25 bags, it is best to inoculate at both ends, but when inoculating, it is not advisable to make this strain very broken, after bagging, you must quickly enter this stove sterilization, at this time when you put this bag, there should be a certain gap between the bags and around the bag, so that the steam can circulate. What is the way to take, the beginning is the rapid heating up of the fire, try to keep it within 4 to 6 hours to raise the temperature to 90 degrees,
At this time, after opening the exhaust hole to put the cold air of the stove away, let it come out of a very hot gas and then close the exhaust valve, and then start recording the time when it heats up to 100 degrees, the sterilization time at 100 degrees is more than 14 hours, you can suffocate overnight, if it is a high-temperature and high-pressure sterilization box, at this time the air pressure in the pot reaches 0.05 mega Pascal when the exhaust valve is opened, the air conditioner can be released and then wait until the pressure drops to 0 megapascals, and then re-increase the pressure Then when the air pressure rises to 0.15 MPascal, it is necessary to maintain it for 3 to 4 hours to meet the sterilization requirements, and after the sterilization is over, it is necessary to wait until the temperature in the pot drops to 60 degrees to 70 degrees, and then the bacteria bag and the bag can be moved directly to the inoculation chamber while it is hot.
Moderator: Okay, Teacher Wang, do you think there are any precautions in this sterilization process?
Teacher Wang: Yes, there are the following aspects of the precautions, when there are the following aspects, we should appropriately extend the time for adding materials, for example, if a material is relatively old and lumpy or found to have mildew spots, this time to extend 2 to 3 hours. The second is to say that if you add material at a time, your amount is relatively larger than 3000 bags, the pile is relatively large, this time is every time you add 500 to 1000 bags, you have to extend it for 2 to 3 hours. The third point is that if the fire is extinguished or cooled down during the process of steaming, you should extend the time as appropriate. The fourth point if you are infected with this bag of miscellaneous bacteria, back to the furnace, back to the pot, at this time you have to extend 5 to 8 hours, because the mixture is a foundation, steaming material it is the key, steaming material to do what fire attack head, fire tail, control this middle, so that under the premise of ensuring this safety, try to increase the temperature in this steaming chamber, a constant, I feel that this is the case, its constant temperature must be above 100 degrees to observe the time of this gas cylinder, You look at this cylinder and it consumes more than 60 kg to 80 kg of water per hour.
Observe this water level, the water level must be maintained at more than half of the time, if you this gas production is large to replenish the water is more difficult, steaming enough 8 to 10 hours after the temperature drops to about 70 degrees, this time quickly move this bag material out, this time is in the process of transportation must be laid on a relatively clean woven bag, maintain hygiene and the bag should be taken lightly, if found that the broken bag should be timely with glue to it sticky. After being transported to this vaccination room, it is necessary to put six or seven layers in a row and a single row, do not lean against the wall, leave a walkway, and wait for the temperature to drop below 30 degrees before starting to inoculation. The purpose of the steaming material is why, it is to achieve thorough sterilization is a very critical part of the success or failure of planting, that is, it is necessary to eradicate the bacteria in this process can not be the fire by the way, or the temperature must be lowered to maintain the temperature.
Moderator: Okay, at the end of the program, I still have to thank Mr. Wang Hua for being a guest of our program, this program ends here, and we will meet again in the next issue.