As a single-chain RNA virus, the mutation of the new crown virus is mainstream. If we know in advance what kind of new coronavirus strain the subject is infected with, it is of great significance to formulate and adjust the epidemic prevention and control strategy and accurately give clinical treatment plans.
On March 16, the team of Professor Gu Bing of the Department of Clinical Laboratory of Guangdong Provincial People's Hospital and the team of Professor Zhang Lei of South China University of Technology published a research paper in emerging microbes & Infections, an authoritative journal in the field of global infection. The study developed a detection method based on RT-LAMP to identify the level of single-base mutation, and designed 2 pairs of specific primers for the conserved region of the original strain gene of the new coronavirus and the specific region of the Delta-specific mutation, which can achieve rapid detection and differentiation of the Delta mutation strain and the wild strain of the new coronavirus under specific circumstances, and clinically verified, and the classification accuracy rate reached 100%.
Establishment of RT-LAMP method for detecting typical mutation sites of COVID-19 Delta
This method realizes the identification of Delta variant strains and real-time monitoring of the infection of the original strains in about 1 hour, and the conventional fluorescence quantitative PCR detection platform can be carried out, which is expected to provide a powerful means for the rapid shunting of patients with suspected new coronary pneumonia and the accurate classification of Delta strains.
Cq-ratio based RT-LAMP technology for the detection and typing of clinical samples of COVID-19
Professor Gu Bing said that the study began in June 2021 and was only published in March this year due to many factors such as the research cycle and the use of manuscripts in journals. "At present, the Delta strain is no longer the global dominant strain, replaced by the Aumechjong strain, but our solution can be quickly copied to the nucleic acid detection of people infected with the Olmiqueron strain."
It is reported that Professor Gu Bing and Professor Li Shan are the co-corresponding authors of the paper, and Dr. Yang Jianning and Dr. Hu Xuejiao are the co-first authors, and the research has been supported by the National Natural Science Foundation of China, the Key Laboratory of Blood Product Quality Control of the State Food and Drug Administration, and the National Key Research and Development Project.
Written by: Nandu reporter Wang Daobin Correspondent Zhang Lanxi Hu Xuejiao