Product catalogs
01
5K 育种家芯片 辅助育种
02
20K 低密度作图芯片 遗传图谱
03
60K medium and high-density chip genetic map
04
120K 高密度芯片 QTL定位
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800K Ultra-High-Density Chip Population Analysis
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Exon capture chip mutation library detection
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Transcriptome sequencing expression analysis/BSR
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Resequencing Population Studies/Low-abundance Sequencing
Pre-saved activities
01
The pre-deposit ≥ 30,000 yuan and the pre-deposit period is more than 3 months
95% off all items
02
The pre-deposit period ≥ 50,000 yuan is more than 3 months
9% off all items
The above pre-stored activities are limited to 2024.10-2024.12
The final interpretation right of the event belongs to the company
Product Introduction
01 - 5K Breeder Chip
Based on 3000 population genetic diversity data of domestic and foreign variety materials, 96 SNP loci were included in the population genetic diversity loci and the SNP marker method for the authenticity identification of wheat varieties. It can be used quickly and effectively for genetic relationship analysis, backcross offspring background recovery rate analysis, variety authenticity verification, etc., and is the most cost-effective low-density chip. In order to better serve wheat molecular breeding, the 5K chip contains more than 2,000 functional marker sites such as published functional genes, QTLs and GWAS loci, covering dozens of genes and markers for disease resistance and agronomic traits.
Fig.1 Publicly reported yield shape loci (Shuanghe Cao et al., 2020)
图2 公开报道的部分农艺性状位点(《The Wheat Genome》,Chittaranjan Kole)
02 - 20K low-density mapping chip
TC-Wheat-Core-20K selected the most genetically representative 20K loci from 5000 wheat germplasm resources worldwide, including 1500 foreign varieties and 3500 domestic backbone parents, historical varieties, landraces and modern varieties. The TC-Wheat-Core-20K assay results provide multi-genome location information to help scientific research work accurately construct genetic maps.
Fig. 3 Distribution of 20K microarray markers on the genome
03 - 120K high-density chips
Agriculture
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Identification of germplasm resources
BSA positioning
Correlation analysis
Genetic mapping
The wheat 120K chip (120K-4HWA) was developed by the team of researcher Jia Jizeng from the Institute of Crop Science, Chinese Academy of Agricultural Sciences and Chengdu Tiancheng Future Technology Co., Ltd. Based on the design of 55K/660K chip sites and more than 5000 domestic and foreign natural population data, including some sites in 55K chips, it is compatible with 55K chip detection data. The microarray achieves a balance in the uniform coverage of the genome and the density distribution of functional genes. 120K-4HWA is one of the most cost-effective chips for accurate identification and functional research of wheat germplasm resources.
Fig.4 Distribution of markers on the genome and gene coverage in the 120K chip
Fig.5 GWAS correlation analysis of the 120K-4HWA chip
04 - 800K ultra-high-density chips
Agriculture
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Group studies
GWAS analysis
BSA analysis
Genetic analysis
The new wheat 800K ultra-high-density chip was jointly developed by Chen Feng's team from Henan Agricultural University and Chengdu Tiancheng Future Technology Co., Ltd. Based on the wheat pan-genome, wheat materials from all over the world were used for analysis, and representative polymorphic loci were selected, mainly covering gene and regulatory regional variation information. The chip is the most dense liquid phase capture probe product currently available, which can meet the various needs of wheat population research. It is especially suitable for population genetic structure analysis, GWAS association analysis, and BSA gene mapping.
Fig.6 Distribution of genomic selection hotspots
Fig.7 GWAS correlation analysis of 800K chips
05 - Exon Capture Chip
Agriculture
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Mutation library sequencing
Group studies
GWAS
BSA analysis
According to the Wheat 10+ Genomes Project, it was found that each modern cultivar has a 3%~4% gene number difference relative to Chinese spring (Walkowiak et al., 2020), and the probe using the reference genome of Chinese spring as a template cannot fully cover this information, especially some translocation line materials obtained through long-term domestication or through some exogenous fragments (e.g., 1BL/1RS transposition line, etc.). Whether it is GWAS analysis of natural population sequencing or large-scale mutant library sequencing, information is lost due to probe background differences.
In order to help scientific research work, the complete genetic variation information can be obtained as much as possible in the sequencing of natural populations and mutant libraries. Based on the wheat exon probe designed in the early stage, the company used the genome sequences of several modern wheat varieties as templates, and used the pan-genome iterative algorithm to design and develop wheat pan-genome exon probes. Launched WheatPanExome products after internal testing and optimization.
Exon capture chip is the best choice for population detection of wheat mutation pool. At present, the sequencing of wheat mutant pool and the mapping of a large number of mutant genes have been reported to use exon capture technology. For example, Jimai 22 mutation library (Yantai University), dwarf anti-58 mutation library (Henan University/Chinese Academy of Agricultural Sciences), Jing 411 mutation library (Chinese Academy of Agricultural Sciences), and Kenong 9204 mutation library (Chinese Institute of Genetics and Development/Hebei Agricultural University) and other mutation libraries were all sequenced and analyzed by exome capture chips developed by Tiancheng Future.
Fig.8 Jing 411 mutation library
Fig.9 KN9204 mutation library
Please contact us for details
Manager Chen: 18215509986
Manager Su: 15928106026
E-mail|[email protected]
Official website|http://www.tcuni.com