What should I do if the oocytes are not spermatozoan?

In the process of doing IVF, I don't know if any expectant mothers have encountered the situation that oocytes are not spermatized, which also leads to many families who have children stuck here without any progress, so how to solve the problem of oocyte sperm in the end? Is there still a chance to have children?

The entry of sperm into oocytes does not necessarily mean that they can be fertilized normally, and it is also necessary to activate oocytes to complete fertilization.

Phospholipase C is located in the apical body and the apical sheath of the sperm, after the sperm contacts the egg for a maxillary reaction, phospholipase C enters to trigger calcium ion oscillation, thereby activating the oocyte, promoting the oocyte to complete the second meiosis, completing the true meaning of fertilization, and initiating sperm-egg fusion and embryonic development.

Spermogenic factors are mainly sperm phospholipase C deletion, abnormal expression, and decreased activity.

Studies have found that phospholipase C-deficient sperm are injected into mouse oocytes together with the phospholipase C gene, and mouse oocytes can be successfully activated.

This suggests that the phospholipase C gene may be an effective oocyte activator in assisted reproductive technologies. However, this technology is still under research and has not yet been applied to the clinic.

Oocyte-derived factors mainly refer to immaturity of oocytes, or abnormalities in some activation signaling pathways within oocytes.

Oocyte assisted activation technology is currently recognized as a means of disposal, and its methods are divided into three categories: mechanical activation, electrical activation and chemical activation:

Electrical activation: That is, the use of the electric field generated by the DC voltage makes the concentration of calcium ions in the egg cytoplasm increase instantaneously, and artificially causes calcium ion oscillations to activate oocytes. However, electrical activation has not been used in clinical practice because its safety cannot be guaranteed.

Chemical Activation: Chemical activation is a method currently commonly used in centers. Chemical activation is the insertion of oocytes into chemical reagents to increase the level of calcium ions in oocytes, artificially causing calcium ions to oscillate, thereby activating oocytes.

Mechanical activation: i.e., slowly aspirate the oocyte cytoplasm with a microinjection needle before injecting sperm to stimulate oocyte activation. At present, mechanical activation is a routine procedure of ICSI, but it is not necessarily effective for oocyte activation disorders caused by genetic defects.