"Twain Supply" The role of Twain in biological experiments

　　Tween is a transliteration of tween, also called Tween emulsifier, which is a type of nonionic detergent for span (sorbitol fatty acid ester) and ethylene oxide condensate. Chemical name: polyoxyethylene sorbitan fatty acid ester, referred to as polysorbate (polysorbate). Since Sipan is an ester formed by sorbitol and different higher fatty acids, Twain actually has the same type of series of products, which are divided into 20, 40, 60, 80 kinds in general fine chemical stores or chemical reagent companies, and are selected according to different needs.

　　Since Sipan is an ester formed by sorbitol and different higher fatty acids, Twain actually has the same type of series of products, which are divided into 20, 40, 60, 80 kinds in general fine chemical stores or chemical reagent companies, and are selected according to different needs.

　　Uses of Twain in biological experiments:

　　1. In biological experiments, emulsifying proteins, when used, tween and the same type of triton x-100 (polyethylene glycol octylphenyl ether) nonionic detergent does not destroy the structure of the protein, can reduce the destruction of the original interaction between proteins. Ionic detergents such as sds destroy the structure of proteins.

　　2. As a blocking agent in biological experiments, the blocking agent should block all unbound sites without replacing the target protein on the membrane, the target protein that does not bind, and the target protein that does not cross-react with antibodies or detection reagents. Tween-20 has the effect of a re-antigen, which can improve the recognition of specificity. When doing western blots, blocking unbound sites on the membrane with an inert protein or nonionic detergent can reduce the non-specific binding of antibodies. The most common blocking agents are BSA, skimmed milk powder, casein, gelatin, and a dilute solution of tween-20 (0.05-0.1%).