Aluminium is the most abundant metallic element in the earth's crust. In acidic soil (pH<5), the main form of aluminum is Al3+, and micromolar concentrations of aluminum ions can destroy plant roots, inhibit plant growth, affect root absorption, and reduce crop yield. About 30% of the world's cultivated land is acidic, and the acidic soil of the mainland is spread across 15 provinces and regions in the south, accounting for about 21% of the country's cultivated area. Aluminium stress is considered to be the second most common abiotic stress after drought.
In the previous research of our group, SlSZP1, an important regulator of protein accumulation of SlSTOP1, a key transcription factor for aluminum stress tolerance in tomatoes, was identified, and the molecular mechanism of SlSZP1 in response to aluminum stress and protection of SlSTOP1 from degradation by SlRAE1 was analyzed (New Phytologist, 2022). The direct effect of aluminum stress on plant roots is the inhibition of taproot elongation, and the mechanism of how aluminum stress inhibits root elongation is still unclear.
近日，Plant Physiology在线发表了中国农业大学园艺学院郭仰东教授课题组题为“Transcription factor SlSTOP1 regulates Small Auxin-Up RNA Genes for tomato root elongation under aluminum stress”的研究论文。 该研究发现Small Auxin-up RNAs (SlSAURs)作为SlSTOP1的靶基因发挥番茄铝胁迫下根系伸长的功能。
In this study, two auxin-responsive genes, Small Auxin-up RNAs (SlSAURs), which respond to aluminum stress and may be target genes of SlSTOP1-SlSZP1, were screened through transcriptome data, and the expression of SlSTOP1 and SlSZP1 was synergistically regulated by yeast monohybrid, EMSA, LUC/REN and other technologies to co-regulate the expression of SlSAURs under aluminum stress by forming a complex. In addition, these two SlSAURs played a role in functional redundancy under aluminum stress and promoted root elongation.
进一步解析其调控根系伸长的机制发现，SlSAURs蛋白在铝胁迫下通过与D-clade Type 2C Protein Phosphatases （SlPP2C.D）互作抑制其磷酸酶活性，进而解除了它们对质膜质子泵（Plasma membrane H+-ATPase）的抑制，促进了质膜质子泵的活性进而促进了铝胁迫条件下根系的伸长。
The study was completed by Guo Yangdong's research group in the College of Horticulture, China Agricultural University. Dong Danhui, a Ph.D. student, and Deng Qilin, a master's student, are the co-first authors of the paper. Professor Guo Yangdong is the corresponding author of the paper. Associate Professor Zhang Na of the College of Horticulture of China Agricultural University and Associate Researcher Zhang Lei of the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences participated in the work. Professor Guo Yan and Professor Yang Yongqing from the College of Biology of China Agricultural University provided some vectors and strains. The research was supported by the National Natural Science Foundation of China and the Beijing Modern Agricultural Industry Technology System.
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