Rhodamine belongs to a class of near-infrared organic dyes, where the absorption and emission wavelengths of rhodamine b, rhodamine 6g, and rhodamine 101 are 500-600 nm in the visible light region. In order to be more suitable for biofluorescence imaging, it can be changed to absorb and emit redshifts by changing its structure and conjugating elements, and then derive a series of *** near-infrared rhodamine dyes, such as si-rodanmin (sir), ge-rhodamine (ge-r) and sn-rhodamine (snr), etc., which can also be subdivided into: sir650 (ex646nm, em660nm), sir680 (ex674nm, em689nm), sir700 (ex691nm, em712nm), etc. Rhodamine dye preserves the good water solubility, high yield and photobleach resistance of traditional rhodamine dye, and integrates the advantages of near infrared, which has been applied to fluorescence imaging of various cells.
Silicon rhodamine dye is a deep red near-infrared fluorescent dye with strong cell permeability and high specificity of DNA probes. With *** photophysical properties, while retaining the superior optical properties of rhodamine fluorescent dyes, the spectrum is significantly redshifted, which meets the requirements of near-infrared fluorescence detection, has good biocompatibility, its excitation wave is usually longer than 600 nm, the emission wavelength is located in the near-infrared region of about 680 nm, compared to the traditional near-infrared cyanotic dye, silyrodamine has good light stability, in biological imaging can obtain a stable and lasting fluorescence signal, can be applied to long-term fluorescence imaging process.
English name: SiR-azide
SiR-N3
Chinese Name: Rhodamine-Azide
Excitation wavelength: 652 nm
Emission wavelength: 674 nm
Molecular Formula: C30H34N6O3Si
Molecular weight: 554.7 g/mol
Storage method: -20 °C, protect from light, avoid moisture.
Structural formula:
Xi'an Kaixin Biological Xiaobian takes you to understand other silicon-based rhodamine dyes:
SiR-azide
SiR-Maleimide
SiR-COOH
SiR-NHS ester
SiR-tetrazine
SiR-Me-tetrazine
SiR-DBCO
SiR-alkyne
SiR-BCN
SiR650-BG
SiR700-BG
The above information is provided by the small editor of Xi'an Kaixin Biotechnology Co., Ltd. ~ ~~