Foreword: Fructooligosaccharides are still very stable at low temperatures of 25 to 5 degrees Celsius, and can be applied to cold foods such as ice cream, fructooligosaccharides are applied to milk powder, which is very useful for detoxification and cleansing of the intestines and two-way regulation of micro-ecological balance. Fructooligosaccharides are used in lactic acid bacteria beverages to solve the problem of lactose tolerance in people and increase the content of water-soluble absorbable calcium. Fructooligosaccharides have received widespread attention from milk powder and food brands because of these characteristics and have been added to various products. But how to test the content of fructooligosaccharides in milk powder? Micro-source detection provides a set of detection methods for the rapid detection of fructooligosaccharides in milk powder by ion chromatography.
method:
Ion chromatography: ICS5000+ matching pulse ampere detector, Au working electrode.
Fructose (100% purity), glacial acetic acid (AR), sodium acetate trihydrate (AR), 50% sodium hydroxide solution (chromatographically pure), fructosanase (derived from Aspergillus niger, enzyme activity of ~25 u/mg), nitrogen (purity≥99. 9%), water (ICW3000), infant formula is purchased from local supermarkets.
Test Results:
Fructose standard chromatogram
The results of the national standard method and the method of this paper are measured
This article refers to GB5009.255-2016 "Determination of Fructosaccharides in Food Safety National Standards", which simplifies the analysis steps and quantitatively analyzes the content of fructooligosaccharides in 6 kinds of infant formula using ICS5000+ ion chromatography. The method is simple, fast, quantitative and accurate, and has good repeatability. The method in this article applies only to sucrose-free foods.