This year's preparation time is not abundant, seeing that January has passed, and February has been inserted into a Spring Festival!
Especially the little white who applied for the national nature for the first time, in order to be promoted, he wanted to apply for the national nature, and he was even more busy......
There are four key time nodes for the application review of the National Natural Science Foundation of China, namely: the preliminary review time, the review time, the centralized acceptance review time, and the outstanding youth review time.
Among the four key time points of the declaring country, the preliminary examination is the most important, mainly to review your format, form, and qualifications, so the tender must be detailed, and the declaration qualifications must be met!
The following are for your reference:
The basic process of project acceptance and review officially released by the NSFC is as follows:
1 national natural bid, the review experts only have 5-10 minutes to review, and the purpose is also very clear: to see if you have the ability to carry out and complete the subject you are involved in smoothly!
The foundation of the preliminary work in the national natural project can be divided into two parts.
In all fairness, if the foundation in the early stage is good enough, the national natural review experts will definitely not be able to give A. So, if the foundation in the early stage is average, is there still a play in declaring the topic?
We all know that the application of National Nature is the art of "asking for money", and we want to express this meaning to the judges: "I can only do this project, and I can do it well, but I am short of money".
Therefore, in order to express their sincerity to the review experts, some applicants have done all the pre-experiments that can be done......
For example, in the research plan, it is said that three cell lines will be used in the future, and all the pre-experiments have been done, the overexpression and silencing of molecule A have been done, a lot of animal experiments have been done, and the luciferase experiments, ChIP and other molecular experiments in molecular interaction are abundant. In this case, experts may feel the need to fund it anymore, as there are not many experiments left to do.
Therefore, if we want to win the favor of the reviewers and enrich the pre-experiments, we can rely on the following remedial measures:
Supplementing some diagrams of the newsletter as the pre-experimental diagram of our bid can not only enrich the preliminary research basis of our application, but also serve as an extension to guide the direction of our future publications.
If you need to link the protagonist molecule to the prognosis of the disease, then using a simple and intuitive univariate/multivariate regression or survival curve is a good choice.
For example, the basic study of tumor monogenic based on biosource analysis is to explore the relationship between specific genes and tumor diagnosis/prognosis/progression and its regulation or mechanism of action. First, we need to explain the clinical significance of screening target disease genes. After all, these are the same tumors, but there is still heterogeneity between them. Here, we need to demonstrate that genes that are meaningful in other diseases are still of interest in the target disease. Usually the specific analysis items that can be combined include expression difference analysis, survival prognosis analysis, univariate multivariate regression analysis, clinical correlation analysis, and single-gene diagnosis accuracy analysis.
Correlation analysis between any two genes is performed based on Pearson, Spearman, or Kendall statistics. This function also supports the calculation of the correlation of normalized results between genes.
Boxplots are used to reflect the central location and dispersion of one or more sets of continuous quantitative data distributions. For example, the comparison of the expression of a single gene between two groups.
If you want to analyze the correlation between the expression of a gene and the tumor grade in a disease, you can also analyze the biograph of a public database.
If you want to analyze the differential expression of a gene between the disease group and the control group, you can solve it with a volcano map or a differential heat map, which is intuitive and clear.
You can also see the gene information that is common to and unique to the combination by looking at the table corresponding to the Venn diagram.
In addition, through the network pharmacological analysis diagram or molecular docking, it is possible to find the target of the TCM compound or the upstream small molecule drug of the protagonist molecule, and show the association between the small molecule drug and the protagonist molecule.
Finally, in addition to bioinformatics, we can also supplement some basic pre-experimental diagrams, such as WB, flow cytometry, electron microscopy, immunofluorescence, and immunohistochemistry to confirm the feasibility and scientificity of our scientific hypothesis.
Therefore, the basic pre-experimental data in the early stage of the national natural bidding must be available, and it must be able to support the scientific hypothesis of the topic, but not too much. So.
That's all for today's sharing, if you have natural related questions, the technical team will provide one-to-one and many-to-one technical support services, and more natural services, welcome to contact us!