Application of pectinase

Pectinase refers to a general term for a variety of enzymes that can break down pectin substances. Many molds and small amounts of bacteria and yeasts can produce pectinase, mainly Aspergillus and Bacilli. Pectinase is roughly divided into pectin hydrolase, pectin lyase (pl), pectin esterase (pe) and proto-pectinase, etc., of which pectin hydrolase is divided into polygalactolipase (pg), polymethyl galactosuronidase (pmg), polylatum plum galactosuronase (rhg), arabinosase, galactase, xylitosyl galactoaldehydease, etc., because people for pg the earliest understanding, the most widely used, so earlier often called pectinase.

Applied Research:

1. Pectinase is applied in feed

The main components of plant cell walls are cellulose, hemicellulose, and pectin. Pectinase can cooperate with cellulase, mannanase, β-dextranase, xylanase, etc. to act together on plant cell walls, through enzymatic cell wall, the nutrients in plant feed can be released, thereby indirectly improving the utilization rate of feed. Reduces the cost of feed and promotes the absorption of feed by livestock and poultry.

2. Pectin disintegrates the cell wall of plant cells

(1) The production of fruit and vegetable juice, the clarification of fruit wine At present, most of the original juices and concentrated juices are used in the production process of that pectinase. Since the specificity of pg is not as high as the esterification requirements of pectin, pg-based enzyme composition is often paid attention to in clarifying juice, and pl is often mainly based on improving leaching juice.

(2) The extraction of natural products, the presence of pectin substances to varying degrees of influence or hinder the release of natural products, under suitable conditions, plant cells will autolysize can also produce decomposition enzymes including pectinase, but this will make the product to be isolated structural changes, and even produce some small molecular by-products that are not conducive to separation in most cases, therefore, relying on the plant cell's own enzyme system is not conducive to the extraction of natural products. In general, the thermal inactivation of the passivation intracellular enzyme system should be carried out, and then the enzyme treatment should be selectively carried out.

(3) Biological degumming of textiles, treated with alkaline pectinase, instead of alkali to cotton and linen and other fabrics for cooking processing and finishing processing, in order to remove the pectin substances of the nascent cell wall clock, in a relatively gentle pH and temperature conditions to make the treated fabric feel soft, high strength, replacing the energy consumption, serious pollution of the traditional hot alkali degumming process. In addition, the degradation of cellulose caused by microbial treatment can be avoided.

(4) The biological pulping of the paper industry, the biological pulping of the papermaking industry is similar to the biological degumming of textiles, which are processed by pectinase and other enzymes to degrade the pectin, hemicellulose and lignin in the raw materials of plant fibers, so that they are decomposed into bundle fibers or single fibers that meet the different requirements of the papermaking industry, so as to produce soft, uniform, elastic high-quality materials. Due to the negative charge of the polymer pectin in the pulp, it can be removed by enzymatic solution below hexasaccharides, avoiding the static phenomenon of finished paper.

3. Partially decomposes the pectin substance in the interstitium of cells

(1) The production of pulp food, pectin in the action of pe to remove methoxy, in the presence of calcium ions to form a gel, thereby maintaining the original shape and hardness of the pulp, as the base of the products are juice, jelly, pulp yogurt, pulp ice cream and so on.

(2) The production of single-cell products, the so-called single-cell products refer to the transformation of biological tissues to form a complete single-cell suspension. The process of enzymatic degradation of pectin substances in the plant cell interstitium to produce a complete single-cell suspension is called leaching. In the process of leaching, on the one hand, endogenous pectinase is inactivated to avoid cell softening; on the other hand, exogenous pectinase is used to moderately degrade extracellular pectin and other components to avoid intracellular substance leakage and reduce quality.