Modern biological fermentation will often use defoamers, today we briefly talk about the combination of temperament for the detection of foam enemy defoamer residues, what are the advantages?
For example, polyether defoamer, which is a non-ionic surfactant, belongs to the block type polyether substances widely used in biological fermentation processes. Such as antibiotic pharmaceuticals, vaccines, recombinant proteins, etc., the advantage is non-toxic but has a strong ability to defoam.
Features are:
Good durability
Non-toxic
Better defoaming effect
Relatively good chemical and thermal stability
Gas chromatography-mass spectrometry, currently widely used in modern analytical testing, involving all walks of life, including biological fermentation drug analysis, pesticide residues, solvent residues, macromolecular analysis, etc., the advantages are high sensitivity, good reproducibility, and easy to operate. Polyether polymer compounds are nonionic surfactants, and in recent years, defoamers have been widely used in the fermentation process, and temperament has been pushed to use.
Temperament detection foam enemy defoamer case:
Micro-source detection used Agilent's 7890b/5977b msd gas combination instrument to develop a method for determining the residue of polyether defoamers in biological fermentation processes. The experimental results show that this method has a high detection sensitivity, and can detect the defoamer with a minimum of 0.05 mg/kg in the sample, and the correlation coefficient r² = 0.9996
Defoamer peaks
Advantages of temperament for residue detection of foam defoamers:
The detection limit and the quantitative limit can be very low, which can effectively reflect the actual residue in the fermentation liquid, and can solve the problem that the liquid equality cannot be solved
The experimental process is simple and efficient, and can be used for large-scale inspection of products
Stability, good reproducibility, can ensure that different production batches and processes caused by interference problems
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