Iceland's main players Gilfi Sigsen and Gumundsen declared that they would not play against England, and the Icelandic national team also issued a statement later.
"Everton's Gilfi West Goodson Burnley's John Berg Bergmondson is no longer able to play in the UEFA League next Saturday in Reykjavik."
Midfielder Gilfi Sigurdson opted to stay at Everton
Former Burns ace John Berger Gudmunson
Iceland coach Eric Hamlen said: "Because of their situation at the club, they chose not to play.
"They have been honest with me and told me about their situation and the reasons for this unfortunate decision.
"They really want to play for Iceland and although it doesn't feel good, they still need to do it.
"Of course, I'm not happy about that. I have to accept it. ”
Sigson, 30, has decided to stay at Everton, who will face Tottenham in the Premier League opener on 13 September.
Goodmundson, 29, opted out of the game, although Burnley's game against Manchester United was delayed.
In addition, some players are unable to return to the national team.
Former Cardiff star Aaron Gunnarsen has been banned by Qatari clubs from travelling under the new FIFA rules.
Alfred Finberggarson of Augsburg was also unable to keep in touch with the team.
Copenhagen ace Ragnar Sigurdsen was injured and unable to land.
Iceland will lose their five best players next weekend.
Former Cardiff midfielder Aaron Gunnarsen
Millwall winger Jon Dudy Bodwasen
Meanwhile England will be keen to avoid being beaten again by Iceland, who were kicked out of the euro 2016.
England assistant coach Freire Alexander said: "Head coach Southgate has done a great job in this squad and they will be coming out with the strongest squad! That's nice! We want to play to their best. ”
But the Situation of the Three Lions is not so optimistic. The game was also a nightmare for Three Lions coach Southgate.
Five of his team members tested positive, though some are expected to recover and prepare to train at St. George's Park on Monday.
Southgate will soon make a final decision on who leaves the team.