Lysine (Lysine) is one of the essential amino acids in the human body, the chemical name is diaminohexanoic acid, and its chemical composition is C6H14N2O2. It has asymmetrical a carbon atom, so there are two isomeric types of L-type and D-type isomeric drugs, which are mainly used as nutrients and as raw materials or intermediates for the synthesis of other products in the pharmaceutical industry, such as reaction with aspirin to form antipyretic analgesic lysine.
Free lysine is easy to absorb carbon dioxide in the air, and it is difficult to prepare crystallization, and general commodities exist in the form of lysine hydrochloride. With the widespread application of lysine, it is necessary to use scientific and effective methods to monitor lysine. Methods for the determination of lysine include high performance liquid chromatography, amino acid analyzer, ninhydrin chromogenic method, etc.
The high-performance liquid chromatography detection method is a commonly used amino acid analysis and detection method in the laboratory, and the samples containing lysine are extracted and purified by appropriate methods. If the sample is a complex biological sample, derivatization may be required. Chromatographic conditions: Select the appropriate column to formulate the mobile phase, typically consisting of water, an organic solvent (such as acetonitrile or methanol), and a buffer, and adjust the pH of the mobile phase to optimize separation and peak shape. Depending on the type of column and detector, set the appropriate flow rate, column temperature, and detection wavelength, and inject the processed sample into the column using an autosampler.
The content of lysine in the sample can be accurately determined by high-performance liquid chromatography, and the chemical drug has the advantages of high sensitivity, high accuracy and good reproducibility. It can be used for qualitative and quantitative analysis of lysine in samples, pharmaceutical quality control, biomedical research, feed content testing and many other fields.
Amino acid composition is an important indicator of biotechnology metabolism research, and the microsource testing laboratory provides the following: Asp aspartic acid, Thr threonine, Ser serine, Glu glutamic acid, Pro proline, Gly glycine, Ala alanine, Cys cystine, Val valine, Met methionine, Ile isoleucine, Leu leucine, Tyr tyrosine, Phe phenylalanine, Lys lysine, His histidine, Arg arginine, Detection services for amino acids such as TRP tryptophan and their metabolites.
Relying on more than 100 instruments and equipment such as spectrum, chromatography, mass spectrometry, energy spectroscopy, and thermal spectroscopy, the microsource testing laboratory is a testing platform under the listed company Titan Technology, which mainly provides testing services in biological, pharmaceutical, chemical and other fields, and the professional R&D engineer team has completed more than 1,000 projects, and is committed to providing efficient and accurate high-quality testing technology services for pharmaceutical companies, bioengineering enterprises, fine chemical enterprises, scientific research institutes and other enterprises. If you have relevant testing needs, welcome to consult!
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