On April 19, we interpreted 15 articles of literature, focusing on: mosquito-borne virus prevention and control, chemical probes, food digestion, laying hens, fruit flies, intestinal physiology, intestinal stem cells, p53, anaerobic biology, Huawei Pharmaceutical, Ma Yinglong Pharmaceutical, TransCode, Palisade Bio.
The daily newspaper was published by R. AI-assisted creation generation, manual review and proofreading.
Cheng Gong/Zhu Yibin/Wang Jinglin et al.Science: Using intestinal bacteria to block the transmission of mosquito-borne viruses
Science——[56.9]
(1) Cheng Gong and Zhu Yibin of Tsinghua University and Wang Jinglin of Yunnan Academy of Animal Science and Veterinary Sciences and their team published important research and found that a mosquito symbiotic enterobacteria distributed in nectar may be used to block the transmission of flaviviruses (a type of mosquito-borne virus), providing a new strategy for the biological control of this type of virus; (2) The researchers isolated 55 culturable bacteria from the intestines of Aedes albopictus in the wild in Yunnan Province, China, among which Rosenbergiella_ YN46 can continue to colonize the intestinal tract of mosquitoes and make mosquitoes resistant to dengue virus and Zika virus infection;(3) Mechanistically, Rosenbergiella_YN46 can secrete glucose dehydrogenase, which converts glucose into gluconic acid when mosquitoes suck blood, acidifying the intestinal lumen, thereby inactivating the viral envelope protein and preventing the virus from invading intestinal epithelial cells;(4) Analysis Rosenbergiella_ The distribution of YN46 in different regions of Yunnan showed that the carrier rate of YN46 in the low-incidence area of Aedes albopictus (52.9%~91.7%) was significantly higher than that in the high-incidence area (0%~6.7%);(5) Under semi-wild conditions, Rosenbergiella_YN46 showed effective cross-age transmission in wild mosquitoes and blocked the transmission of dengue virus through new adult mosquitoes.
【Original Information】
A naturally isolated symbiotic bacterium suppressesflavivirus transmission byAedesmosquitoes
2024-04-19, doi: 10.1126/science.edn9524
How to use chemical probes to explore the extreme environment of mosquito larvae gut?
Journal of the American Chemical Society——[15]
(1) the development of a novel chemical probe to study and control the extreme alkaline intestinal environment of mosquito larvae to prevent mosquito-borne diseases;(2) the protective triazabutadiene (pTBD) in the larval gut is unprotected under alkaline conditions and releases aryl diazoide ions (ADIs), resulting in protein modification;(3) Through copper-catalyzed click chemistry, the terminal alkyne group of the probe reacts with an azo fluorophore, enabling quantitative detection on SDS-PAGE and visualization under fluorescence microscopy;
【Original Information】
Chemical Probes to Interrogate the Extreme Environment of Mosquito Larval Guts
2024-03-14 , doi: 10.1021/jacs.3c14598
Qi Bin's team Nature sub-journal: Bacterial peptidoglycan activates host food digestion and improves natural adaptability
Nature Communications——[16.6]
(1) Qi Bin's team from Yunnan University published an article using a nematode model to find that bacterial peptidoglycan (PGN) is a signal that initiates food digestion and improves the natural adaptability of nematodes;(2) Screening of E. coli mutants found that PGN is a unique food signal that can activate nematodes to digest inedible food;(3) The glycosylated protein BCF-1 in the gut interacts with bacterial PGN and inhibits the mitochondrial unfolded protein response (UPRmt) by regulating the release of neuropeptide NLP-3;(4) Activation of UPRmt hinders food digestion, which relies on the innate immune p38 MAPK/PMK-1 pathway, and inhibition of PMK-1 can alleviate the digestive defects of bcf-1 mutants;(5) Digestive defects of bcf-1 mutant nematodes have reduced natural adaptability;(6) The study revealed that PGN-BCF-1 interaction acts as a "signal to trigger food digestion", improving the food utilization rate of animals in nature, thereby enhancing animal adaptability.
【Original Information】
Bacterial peptidoglycan acts as a digestive signal mediating host adaptation to diverse food resources in C. elegans
2024-04-16 , doi: 10.1038/s41467-024-47530-y
Yunnan Agricultural University: Turning waste into treasure! Mulberry branch fiber helps improve the quality of laying hens
Microbiome——[15.5]
(1) Pan Hongbin and Huang Ying of Yunnan Agricultural University and their team found that mulberry fiber (MF) as a feed additive can reduce the liver fat of laying hens and improve the fatty acid composition of egg yolk through the gut-liver axis, which is beneficial to both chickens and eggs;(2) The addition of 5% MF to the feed reduces the fat deposition in the liver of laying hens, and does not adversely affect egg production performance and egg quality;(3) MF increased the content of polyunsaturated fatty acids in egg yolk, changed the diversity and composition of intestinal microbiota in laying hens, and increased the abundance of genes involved in the biodegradation of fatty acids and the synthesis of short-chain fatty acids in the intestinal microbiota of laying hens;(4) There was a significant correlation between the metabolic enzyme activities of liver lipase, lipoprotein lipase and total esterase in laying hens and the intestinal microbiota, including negative correlation with the diversity of intestinal microbiota and multiple correlations with specific intestinal flora and viruses;(5) Liver enzyme activity and intestinal microbiota composition directly affect liver fat content, and liver enzyme activity and intestinal microbiota diversity have a significant effect on egg yolk fatty acid composition.
【Original Information】
Mulberry branch fiber improved lipid metabolism and egg yolk fatty acid composition of laying hens via the enterohepatic axis
2024-04-12 , doi: 10.1186/s40168-024-01788-Y
Nature: Drosophila gut neurons discover new sodium receptors
Nature Metabolism——[20.8]
(1) Drosophila was able to sense sodium ions independently of the taste system through intestinal neurons after salt deficiency, revealing a taste-independent sensing mechanism to maintain sodium balance;(2) Drosophila Ir76b mutants were able to respond to sodium after salt deficiency, indicating the existence of sodium sensing pathways outside the taste system;(3) By screening the desired cells to induce sodium preference, a group of internal sodium sensing (INSO) neurons located in the foregut were found to be essential for guiding sodium preference behavior;(4) INSO neurons primarily innervate the intestinal epithelium through their dendritic processes and project their axons along the esophagus to the large abdomen, with a specific immediate response to sodium ions;(5) It is important to note that sodium-induced responses are observed only after a period of sodium starvation.
【Original Information】
Postprandial sodium sensing by enteric neurons in Drosophila
2024-04-03 , doi: 10.1038/s42255-024-01020-z
Domestic team Nature: Genetic decoding of intestinal cell plasticity in Drosophila
Nature Communications——[16.6]
(1) Chenhui Wang from ShanghaiTech University and Rongwen Xi from the Beijing Institute of Biological Sciences, together with their team, found that removing the Tramtrack transcription factor from differentiated intestinal epithelial cells (ECs) can initiate Prospero-mediated cell translineage and differentiation to form cells similar to enteroendocrine cells (EEs) by establishing a genetically traceable in vivo model; Removal of Prospero transcription factors from differentiated EEs results in the loss of EE-specific transcription programs and acquires the identity of intestinal progenitor cells, allowing cells to re-enter the cell cycle or differentiate into ECs;(3) Gut progenitor cells, ECs, and EEs have similar chromatin accessibility profiles, which supports the idea that cell plasticity is enabled by a combination of pre-existing chromatin accessibility and switchable transcription programs;(4) Genetic analysis of in vivo genetic models revealed that NuRD chromatin remodeling complexes, cell line conflicts, and age were barriers to EC-to-EE transgeneration.
【Original Information】
Cell-fate conversion of intestinal cells in adult Drosophila midgut by depleting a single transcription factor
2024-03-26, doi: 10.1038/s41467-024-46956-8
Huanong Cell: Countercurrent stimulates stem cell proliferation and promotes intestinal homeostasis in Drosophila
Cell Reports——[8.8]
(1) Li Xiaoxue and her team from Huazhong Agricultural University published a study revealing a novel mechanism by which reactive oxygen species generated by Duox activation in Drosophila Malcolt promote intestinal epithelial cell renewal through the counterflow system;(2) Drosophila gut-specific silencing of Duox did not affect stem cell turnover, but after silencing Duox in Malcolski main cells, stem cell turnover was significantly reduced, and the ROS produced by Duox activated the expression of the JAK-STAT pathway ligand cytokine upd3;(3) Intestinal stem cell turnover was reduced by upd3 silencing, but ROS was not the main molecule causing intestinal injury after infection;(4) Intestinal infection activated the host to produce intestinal reflux from the duct to the anterior midgut, and then formed an aquaporin Drip-dependent aquaporin, and silencing the drip gene inhibited the formation of intestinal reflux and intestinal stem cell regeneration;(5) This study not only elucidated the role of Duox in intestinal homeostasis, but also described the reverse fluid flow in the gut for the first time, providing an interesting illustration for organ-to-organ communication.
【Original Information】
Duox activation in Drosophila Malpighian tubules stimulates intestinal epithelial renewal through a countercurrent flow
2024-04-11 , doi: 10.1016/j.celrep.2024.114109
Nature: Novel epigenetic mechanisms of intestinal stem cell self-renewal and differentiation
Nature Communications——[16.6]
(1) This study revealed a novel epigenetic mechanism by which Drosophila intestinal stem cells maintain the balance of self-renewal and differentiation, namely, Polycomb Repressive Complex 2 (PRC2) to maintain intestinal stemness in Drosophila by silencing miR-8, and (2) Enhancer of zeste "E(z)" by RNAi knockdown or gene knockout enzyme subunit Enhancer of zeste Mediated loss of PRC2 activity in intestinal stem cells (ISCs), resulting in stemness loss and precocious differentiation of enteroblasts into intestinal epithelial cells;(3) Mechanistically, E(z)/PRC2-mediated lysine trimethylation of histone H3 at position 27 (H3K27me3) targets miR-8, revealing the dynamic relationship between E(z), miR-8 and Notch signaling to control stemness and differentiation of ISCs;(4) These findings provide a new perspective on the epigenetic regulation of stem cell norms, which is of great significance for maintaining intestinal homeostasis.
【Original Information】
Polycomb-mediated silencing of miR-8 is required for maintenance of intestinal stemness in Drosophila melanogaster
2024-03-02, doi: 10.1038/s41467-024-46119-9
Nature: How does p53 protein help intestinal stem cells regenerate after radiation injury?
Nature Communications——[16.6]
(1) In this study, we found that the p53 protein promotes the formation of intestinal regenerative stem cells after severe radiation injury, thereby contributing to the recovery of intestinal epithelial cells in mice;(2) the p53 target gene is especially enriched in regenerative epithelial cells that undergo fetal-like reversal, such as resuscitation stem cells (revSC) that promote animal survival after severe injury to the gastrointestinal tract;(3) In mice with p53-specific deletion in the gastrointestinal epithelium, radiation cannot induce fetal-like revSC;(4) Transient p53 expression is required for induction of revSC, and this process is regulated by Mdm2-mediated negative feedback;(5) This study suggests that p53 inhibits severe radiation-induced gastrointestinal injury by promoting fetal reprogramming of irradiated intestinal epithelial cells.
【Original Information】
p53 promotes revival stem cells in the regenerating intestine after severe radiation injury
2024-04-08, doi: 10.1038/s41467-024-47124-8
Cell: Unraveling the intestinal microstructure: the formation and renewal mechanism of villous smooth muscle
Developmental Cell——[11.8]
(1) Single-cell mapping of intestinal development trajectories was constructed by single-cell RNA sequencing and quantitative lineage tracking of mouse gut;(2) Intestinal villous smooth muscle cells were derived from the transformation of local fibroblasts to myofibroblasts, and TNC+ SMA+ myofibroblasts were the precursor cells of embryonic and adult lymphoid smooth muscle;(3) The NOTCH3-DLL4 signaling pathway controls the assembly of smooth muscle fibers with adjacent chyle ducts and is essential for fat absorption;(4) The results of this study provide new cellular and molecular targets for accelerating intestinal repair and recovery of digestive function.
【Original Information】
Villus myofibroblasts are developmental and adult progenitors of mammalian gut lymphatic musculature
2024-03-26 , doi: 10.1016/j.devcel.2024.03.005
"Anaerobic Biological" intestinal formula drug KAL-002 obtained clinical approval
(1) On April 17, the clinical trial of KAL-002, a Class 1 innovative drug for therapeutic biological products, was approved by Sichuan Anaerobic Biosciences, and a phase 1 clinical trial will be launched soon;(2) KAL-002 became the first intestinal formula drug approved for clinical use in China, which will be used for the treatment of chemotherapy-associated diarrhea and radiation enteritis;(3) The drug is a multi-group live bacterial formula composed of 5 strains, which can reduce the frequency of diarrhea, improve the degree of abdominal pain, and improve the quality of life of patients;(4) Sichuan Anaerobic Biology has more than 2,000 human microbial strain resources, and has mastered the core technology of the whole chain from bacteria to medicine.
【Original Information】
Sichuan Anaerobic Biotechnology obtained the first implicit license for clinical trials of intestinal formula bacteria drugs in China|Daotong Family
2024-04-17 , Daotong Investments
Huawei Pharmaceutical's posaconazole enteric-coated tablets were officially approved
(1) On April 16, Huawei Pharma announced that its posaconazole enteric-coated tablets were officially approved by the State Food and Drug Administration;(2) Posaconazole enteric-coated tablets are a kind of ergosterol synthesis blocker, which belongs to the second-generation triazole antifungal drugs, which are clinically used for the prevention and treatment of invasive Aspergillus and the prevention of Candida infection;(3) Posaconazole enteric-coated tablets are marketed in the United States, Japan and many countries in the European Union, and the original Merck imported products are listed in China, and 3 generic drugs are on the market;(4) According to the data of Yaozhi.com, the sales of posaconazole enteric-coated tablets in domestic hospitals in 2022 will be 60 million yuan, a year-on-year increase of 85%, and the market growth rate is fast.
【Original Information】
Posaconazole enteric-coated tablets are officially approved!
2024-04-16 , Huawei Pharmaceutical
Ma Yinglong Pharmaceutical's "Compound Polyethylene Glycol Electrolyte Powder" obtained the drug registration certificate
(1) On April 17, Ma Yinglong Pharmaceutical Co., Ltd. announced that its compound polyethylene glycol electrolyte powder (III) was approved by the State Food and Drug Administration to obtain the drug registration certificate approved by the State Food and Drug Administration;(2) Compound polyethylene glycol electrolyte powder (III) is a prescription drug, which is suitable for colorectal endoscopy and intestinal content removal before colorectal surgery;(3) The drug was first approved for marketing in France in 1987, and the State Food and Drug Administration approved the original research and import marketing in 2014;(4) According to the data of Minenet, the domestic market sales of compound polyethylene glycol electrolyte powder will reach 1.518 billion yuan in 2022, of which the sales of compound polyethylene glycol electrolyte powder (III) will be 206 million yuan.
【Original Information】
Ma Yinglong Pharmaceutical Group Co., Ltd. Announcement on Obtaining the Drug Registration Certificate
2024-04-17 , Shanghai Securities News
TTX-MC138, a Phase 1/2 clinical trial for TransCode's advanced solid tumors, was approved
(1) On April 15, TransCode Therapeutics announced that it received FDA approval for a Phase 1/2 clinical trial of TTX-MC138, an investigational pipeline of TTX-MC138, (2) a trial designed to evaluate the safety and preliminary anti-tumor activity of TTX-MC138 in patients with advanced solid tumors, (3) a Phase 1/2 clinical trial consisting of an initial dose escalation study and a dose expansion study in patients with multiple metastatic solid cancers, (4) TTX-MC138 is believed to be a candidate for the treatment of colorectal cancer, A variety of solid tumors, including breast cancer and pancreatic cancer, have been shown to significantly reduce tumor burden and prolong survival time.
【Original Information】
TransCode Therapeutics Announces FDA Clearance To Initiate Phase 1/2 Clinical Trial with TTX-MC138 in Patients with Advanced Solid Tumors
2024-04-15 , TRANSCODE
Palisade Bio UC drug PALI-2108 was evaluated positively in vitro
(1) On April 16, Palisade Bio announced that its pipeline drug for the treatment of ulcerative colitis (UC) PALI-2108 has obtained positive results in vitro activity evaluation experiments;(2) PALI-2108 is a colon-specific phosphodiesterase-4 (PDE4) inhibitor prodrug;(3) PALI-2108 has been shown to be effectively converted to active PDE4 inhibitor form within 24 hours in the feces of healthy and UC volunteers, with an average conversion rate of 90.1%;(4) The results suggest that PALI-2108 has the potential to be a novel targeted therapy for the treatment of patients with moderate to severe UC.
【Original Information】
Palisade Bio Successfully Demonstrates Bioactivation of PALI-2108 In Ex-Vivo Study of Normal Healthy and Ulcerative Colitis Patients’
2024-04-16 , Palisade Bio
感谢本期日报的审核者:mildbreeze,九卿臣,章台柳,RZN,圆圈儿,Richard
Click here to read the daily reports for the past 10 days:
0418 | 聚焦特殊人群：母婴肠道菌群与健康
0417 | High-score Nature sub-journal: Bringing together the power of global players to help intestinal microbiome research
0416 | Multi-article focus: To protect the gut, no one can do without immune cells and microflora
0415 | Cell Blockbuster Review: Systematic Analysis of Cancer Complexity, the Role of Microbiota Should Not Be Underestimated
0414 | A review of the high-scoring Cell sub-journal, an article about fermented foods
0413 | 3 major articles in the sub-journal to analyze the current situation and future of microbiome therapy
0412 | The 10 articles focus on the new achievements of the domestic team, and the research on intestinal and microbiota has blossomed in many places
0411 | Nature Today: How do colon cells work with T cells to fight off bacterial infections?
0410 | 今日Cell：首个大规模泛癌转移瘤菌群图谱重磅出炉
0409 | 高分Nature子刊：菌群产物药物研发取得新进展