Wheat is one of the world's major food crops and one of the continent's most important food crops. The emergence of wheat molecular breeding technology has accelerated the process of wheat breeding, which can effectively improve the quality, yield and adaptability of wheat, and is one of the important means of wheat breeding research.
GenoBaits® Wheat 100K Panel products rely on more than 1,000 hexaploid wheat resequencing data, 45,000 GBS data, nearly 1,000 exon sequencing data and commercial chip data at home and abroad, and cover thousands of QTL sites, suitable for wild species, farm seeds, cultivars and diploid and tetraploid germplasm materials, and can be used for germplasm resource evaluation, map construction and genome-wide association analysis. In the product development process, high-quality SNP sites in foreign and domestic materials were selected respectively, and germplasm resources of 100KSNP were generated through site optimization, and the initial probe was designed to use 100 materials at home and abroad, and finally the product was formed after experiments and optimization. Among them, the high-quality SNP data in foreign materials came from 1,000 wheat exomes project, dbSNP database, GBS database, CropSNPdb, etc.; High-quality SNP data from domestic materials come from WGVD: wheat database, wheat genome variation joint database, etc.
Product features and advantages
1. High representation and rich diversity information
Relying on more than 1,000 hexaploid wheat resequencing data, 45,000 GBS data, nearly 1,000 exon sequencing data and commercial chip data at home and abroad, and covering thousands of QTL sites, it is suitable for wild species, farm seeds, cultivars and diploid and tetraploid germplasm materials.
2. Tag segments for greater information
This product is 100K labeled segments, which can actually provide up to 251,215 SNP site information, which ensures the requirements for the number of sites in scientific research and application, and greatly reduces the cost of use. The chip contains 128 functional labels, covering 21 common traits in wheat, including 63 disease resistance markers, 15 quality trait markers, and 20 yield trait markers.
3. Wide range of applications
It can be used for germplasm resource evaluation, map construction, genome-wide association analysis, molecular marker-assisted backcross breeding and genome selection.
Product application direction
scientific research
▶ Genetic map construction
▶QTL analysis
▶ Genome-wide association analysis (GWAS)
Breeding applications
▶ Genotype identification of germplasm resources
▶ Genome-wide selection breeding
▶ Genetic similarity analysis, kinship analysis
▶ Breeds protection, authenticity identification
Product service process
1. Send samples for quality inspection
2. Capture and build a library
3. High-throughput sequencing
4. Data quality control
5. Cloud bio-information analysis
Product data performance
△Figure 1 Distribution of wheat 100K liquid chip markers on chromosomes
In Figure 1, the 108,836 core SNP markers of this product are evenly distributed on the wheat chromosome
*Ruler Definition: The number of core SNPs in each sliding window (window step 1Mb) on the genome
△Figure 2 Statistics of mSNP-tagged segments of wheat 100K liquid chip on chromosomes
△Figure 3 MAF distribution map of core SNP sites of wheat 100K liquid chip
△Figure 4 Uniformity and detection rate of core SNP sites of wheat 100K liquid chip
△Figure 5 Annotation statistics of core SNP sites of wheat 100K liquid chip
△Figure 6 Detection rate of core SNP site samples of wheat 100K liquid chip chip
Braidi wheat species series products