Glycine (NH₂CH₂COOH), also known as aminoacetic acid, is a simple structure of the amino acid. The appearance is white crystalline or crystalline powder, as an important fine chemical synthesis intermediate, can be used for the synthesis of porphyrin, purine, creatine and other pathways, widely used in pesticides, medicine, food, biochemistry and other fields. How can glycine content in dairy products be detected using ion chromatography?
Experience has shown that ion chromatography has strict requirements for the incoming sample, and requires the input sample to remove organic matter, macromolecular substances, proteins and other substances. Some of the water-soluble proteins in the non-hydrolyzed sample solution could not be removed even after high-speed centrifugation, so the micro-source laboratory consulting the literature finally decided to use a protein sedimentant to remove the water-soluble proteins. The experimental process is as follows:
Accurately take 3.0g of liquid dairy samples in a volumetric flask, add 3mL of potassium ferrocyanide solution and shake well, then add 5mL of zinc acetate solution to precipitate the protein. Then add ultrapure water to the scale shake well, let stand for 0.5h to protein layering, filter out the upper layer of grease with quantitative filter paper, and discard the first 5mL of primary filtrate. Take 5 mL of filtrate and place it in a centrifuge tube, and finally perform the centrifugal operation using a high-speed centrifuge and wait for the next operation.
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Columns
AminoPac—PAl0 separation column and protection column (2mm)
electrode
Au electrode
Column temperature
30℃
Eluent
A is ultrapure water, B is 250mmol/LNaoH.C is a concentration of 1mol/L sodium acetate
velocity of flow
0.25mL／min
Injection volume
10μL
Spectra of samples containing free glycine
The determination of glycine by this method does not need to undergo derivatization treatment, and is directly analyzed on the column, which simplifies the operation steps and simplifies the entire experimental process and shortens the detection time. Good accuracy and high sensitivity, suitable for rapid analysis of glycine in production.