A woman in the United Kingdom wants to get pregnant and have a child, but she does not want to find an irresponsible boyfriend, of course, she does not want a one-night stand or other riotous operations, so she contacted the hospital for artificial insemination, and found that the cost is too expensive!
In order to save money and decide DIY, I found a satisfactory sperm through the sperm donation platform, and then purchased a kit such as a syringe, and through the video teaching of YouTube, I actually got pregnant once, which made the doctor stunned! It's too hardcore, it's a blessing, how exactly is it achieved?
DIY artificial insemination, how exactly is it achieved?
Stephenie Taylor, who lives in Nunthorpe, Teeside, England, is a mother who has a 5-year-old son, Frankie, but she desperately wants to give her son a sister, according to the British newspaper The Sun.
However, Stephanie, who has been hurt by her ex-boyfriend, does not want to have a new relationship, because in case it is not appropriate, her son Frankie may be hurt again, so she began to seek a clinic that can operate artificial insemination, but the best price also needs 1600 pounds (14128.96 yuan), which is also a large expense for stephanie who is more financially strapped.
So Stephanie began to look for artificial insemination information, the result really turned to a lot of methods on how to artificial insemination, and there are corresponding tools and materials to introduce, Stephanie bought the relevant equipment and tools according to the tutorial, according to the tutorial guidance step by step operation, actually a success, a few days ago has successfully given birth to a baby girl.
Stephanie tasted the sweetness, and she even planned to have three or more more, and the anonymous sperm donor also said that he could donate sperm again if needed.
DIY artificial insemination operation, can it really be achieved?
To understand the difficulty of artificial insemination, it is necessary to understand the process of natural fertilization, because this life is born at the beginning, it is the most wonderful and magical journey! In the process of sperm being released to fertilizing an egg, there are several difficulties to go through, and it will probably go through the following process:
1, healthy men excrete up to hundreds of millions of sperm at a time, some will be more, but some cases will be less, there are a large number of "disabled" sperm, these sperm asymmetry, or poor swimming ability, generally if the proportion of healthy sperm is less than 50%, it will have an impact on the outcome of conception;
2, the other is that the semen is very viscous when discharged, but it will soon liquefy, and a large number of female endocrine are washed away, and the remaining part will start to the depths of the female body;
3. After the sperm passes through the cervix to the uterine cavity and comes into contact with the endometrium, there will be a very important process, called sperm energization, the endometrium will produce α, β amylase to relieve the "de-energization factor" on the parietal enzyme of the sperm, so that the sperm obtain the ability to inseminate, called the ability of sperm to obtain;
4, painstaking sperm finally arrived at the egg, at this time the exposed apical enzymes released, dissolve the zona pellucida and make the sperm through the channel into the plasma membrane of the egg, and finally reach the egg cell, after fertilization of the egg peripheral formed a new protective layer, no longer accept the sperm arrived.
The above is the process of natural fertilization, so artificially inseminated sperm can save N more troublesome procedures and directly let the sperm reach the egg, but there needs to be a very important process, that is, "artificial sperm energization", and then accurately deliver the egg for fertilization. So how exactly is the artificial insemination process done?
The process of artificial insemination
Artificial insemination is the use of artificial rather than natural mating methods, the semen into the female uterus or cervix of the insemination process, in general, artificial insemination has the following methods:
1. Intravaginal Insemination (IVI)
2. Intracervical Insemination (ICI)
3. Intrauterine Insemination (IUI)
4. Intratubal Insemination (ITI)
It is estimated that the name definition also knows these methods, the simplest way is IVI and ICI, the former only needs to be sent into the vagina, the latter to the cervix, because it will also pass through the endometrium, so it will be energized through natural channels.
However, the way of ICI and IUI can also make it easier for sperm to be energized in the endometrium by means of elution, of course, the more sperm can be obtained, the probability of fertilization is also higher. Detergent is actually a popular saying, its intention is to isolate healthy sperm as much as possible, of course, it can also deal with the semen of the virus in virus carriers such as AIDS, hepatitis B, hepatitis C, syphilis and so on.
The male semen is separated from other substances such as white blood cells, semen, etc. in a special way (there are simple washing methods, direct upstream methods and discontinuous density gradient methods, etc., the sperm quality obtained is the lowest, the latter is the highest), the virus is separated from other "impurities", and it generally takes 2 hours to obtain healthy sperm, which can obtain a high proportion of sperm with normal morphology and strong motility.
But in the position of artificial insemination in the fallopian tubes does not pass through the endometrium, the need for in vitro energy, in theory this way of sperm path is the shortest, but in fact because of its special invasiveness, because the fallopian tubes are very fragile, so it is the least used, the most commonly used is ICI, because it is the simplest, but the IUI artificial insemination method after lavage is significantly higher.
Which method did Stephanie use?
The Daily Star does not say which method Stephanie used, but it is speculated from the process that the ICI method (intra-cervical artificial insemination), that is, the use of sterile syringes (syringes) to send sperm directly into the cervix, and through natural empowerment, natural conception is the most likely, because the IUI method generally requires washing:
Elution method 1: Simple centrifugation, collect pelleted large sperm clumps, but mixed with dead sperm and other cell debris;
Washing method 2: According to the swimming ability of sperm, take sperm with strong motility in the upper layer, which can be taken multiple times;
Elution method 3: Placing semen above different density gradient culture solution, separating the most energetic sperm through the gradient distribution solution after centrifugation, and does not contain other cells, this method is the best quality, but the cost is high, and the disposal is also troublesome.
But even if it is a general centrifugal rotation separation environment, Stephanie should be difficult to obtain, the author speculates that after getting the sperm donor's semen, the separation part is directly injected into the cervix through a sterile syringe, of course, it may also be vaginal, after all, as long as the sperm vitality is enough, it can be conceived normally.
Therefore, this method of artificial insemination is really not very difficult, of course, there are now "artificial insemination" that have changed the flavor! But don't think that artificial insemination is very tolerant, because IUI and ITI are more difficult, and the requirements for implantation in the uterus after in vitro in vitro insemination (IVF) are higher.
Further reading: When is fetal sex determined?
As we all know, female chromosomes are only X-type, and males are X and Y-type, if XX is female, if XY is male, theoretically, it is decided at the moment when sperm and egg combine, isn't it?
But in fact, it is not, at least not all! Before 6 weeks of gestation, the fetus is still sexless! At this time, the fetus only has a reproductive crest and two catheters in the body, which can form the testicles and can also form the ovaries! When the embryo is an XY chromosome, then there is an SRY gene on the Y chromosome that induces the formation of the testicles, that is, boys, and the XX chromosome, because there is no SRY gene, will develop ovaries at 10-11 weeks of gestation, that is, girls.
However, there are moth things in the middle, such as the Enh13 gene difference in the embryo will still change the sex, the real factor among the regulatory genes SOX9, SOX9 gene has three enhancing factors: eSR-A, eSR-B, eALDI, in which the difference will determine the final sex.
For example, XX combines to develop into a woman, but if the three enhancement factors are expressed at a high level, so that SOX9 is expressed at a high level, the original ovaries will disappear into the testicles, or XY men will appear ovaries, so there are not only two male and female sexes, but there are many kinds, which sounds terrible but is a fact.