1. Selection of cultivated water sources
In order to ensure the standard of high quality and sustainable breeding of algae liquid, please use tap water (24 hours of volatile residual chlorine gas in light exposure storms) or clean underground fresh water (no precipitation or discoloration of water bodies in light storms for 12 hours); never use pond water, surface water or mountain spring water and other water sources that contain more weeds and bacteria and microorganisms or copepods;
2. Introduction to inoculation of cultivation process:
Chlorella cultivation must be inoculated on sunny mornings, and must be kept sunny for three days after inoculation (pay more attention to the weather forecast when cultivating algae liquid);
If special circumstances are in a hurry to use and need to be cultivated in rainy weather, refer to the second point of light requirements;
Inoculation sequence: add 50 kg of water to the container, first put in the powder preparation, on the basis of full dissolution, and then add liquid in turn (the order of addition of liquid preparation is not required in order), then add 40 kg of water, and add 10 kg of algae liquid, a total of 100 kg;
After the algae liquid is fully stirred evenly, it is then filled into a transparent container or a transparent bag, and the mouth of the bag is tightened or the cap is tightened;
The amount of water in the dissolved medium is as much as possible, and the dissolution will be more rapid and fully uniform;
It is never possible to dissolve the medium after the algae seeds have been added;
After the algae liquid is matured, 10 catties of algae seeds are retained first, and the others are then put into use;
3. Light requirements during cultivation:
L:D guarantees a ratio of 12:12, and the optimal light ratio of 14:10 can improve the proliferation rate of algal fluid; the proportion time is especially based on indoor light irradiation cultivation as an implementation requirement;
If the cultivation is rainy weather, it is necessary to use 45 watts of three primary colors 6500K fluorescent lamps to illuminate the cultivation of daylight color cold light source, so as to avoid the failure of algal liquid cultivation caused by insufficient light or the loss of algae liquid and the degradation of algal liquid quality caused by the dark and insufficient activity of algal fluid;
If small containers are used for cultivation, such as 1 liter (2 kg) containers and below, the distance of the bulb irradiation 2 days before cultivation is about 12 cm from the algae liquid container, and the distance from the future is about 10 cm, and the bulb irradiation distance shall not be less than 8 cm;
If the use of large container cultivation, greater than (including) 2.5 liters (5 kg) and above, the light source distance can be adjusted to 6-10 cm, not less than 5 cm distance; the use of transparent bags to cultivate the light distance is the same as the requirements of large containers, each square can be set up 2-4 bulb ceiling irradiation;
In outdoor breeding, the light is based on the natural sunrise and sunset, and the maximum placement can be placed in the place where the light can be received for the longest time per day; the alternating spring and summer season to the late autumn season (southern region), when the outdoor sunny day, the temperature is stable during the day and lasts above 25 degrees, it is necessary to cover the shade net or put the algae liquid in the shade of the tree all day or noon and pay attention to whether the algae liquid is overtemperature [Note: it is the temperature of the algae liquid, not the temperature]; other seasons or time periods are operated according to the actual situation, if the noon light is more intense, Then take shading measures to avoid strong light exposure;
In this point, the cultivated bulb is used to try to follow the required wattage, because it is impossible to measure the other three-color wattage bulbs one by one, and the specific light intensity cannot be given; if it is necessary to replace other three-primary color bulbs with large wattage or small wattage, it is necessary to know the value of the distance and light intensity;
45 watt tri-primary color 6500K fluorescent lamp daylight color cool light
4. Selection of cultivation container:
Chlorella cultivation containers need to choose a fully transparent material (exactly the same as the photosynthetic bacteria cultivation material) barrel or plastic bag can be;
When using the container to cultivate algae liquid, do not fill the container with algae liquid, please leave 1% of the air volume, and then tighten (tie) the container;
After inoculation, tighten the cap or tighten the mouth of the bag and shake it 2-4 times a day to help the precipitated algae liquid float up to obtain light to increase breeding;
If you are busy, you can place a circulating device in the container, the wattage of 8-15 watts can be (the wattage can not be too high, so as not to centrifuge too high to cause the destruction of algae cells), at 8 a.m. or 14:00 p.m. two time periods are opened for 1 hour or more, to the overall algae liquid can be recycled as the standard; try to manually shake the algae liquid as the first choice;
The container is selected as a transparent plastic drum or a transparent plastic bag, which is consistent with the photosynthetic cultivation container
5. Temperature requirements of algal liquid during cultivation
Chlorella cultivation temperature is in the range of 20-30 degrees, the optimal temperature of algae liquid is between 25-30 degrees, not more than 32 degrees; the cultivation temperature is directly related to the proliferation density of algal fluid, maintained in the range of 25-30 degrees, the proliferation density of algae liquid and other indicators will reach the best;
6. Time requirements for algae liquid cultivation
Algae liquid cultivation cycle is 3-5 days, algae liquid becomes deep and green can be put into use or seed retention, on the basis of light and temperature is appropriate, the breeding cycle is generally 4 days; if the whole process uses bulb irradiation for breeding, the temperature is suitable, then extend the time of one day, to breed 5 days is better;
7. Storage requirements after the algae liquid is mature:
Cultivate mature chlorella, if you need to keep seeds, place them in a cool dry and dark place for 2 months, and the storage environment temperature should not be higher than 32 degrees;
If long-term preservation of algae seeds is required, the mature algae seeds are placed in the refrigerator within 2-10 degrees celsius, and the storage cycle can be extended to 6 months to more;
If the amount of single incubation is large and needs to be preserved, please refer to the first requirement (the lower the storage environment temperature, the longer the corresponding storage period);
Chlorella stored for a long time in summer (stored in a non-refrigerator preservation environment), algae liquid may appear odor and other situations (chlorella cells are plant proteins, which will appear odorous with the extension of the preservation cycle), as long as the storage cycle is not exceeded, it can be normally put into breeding and use;
The normal phenomenon of layered precipitation will occur in the algae liquid with a long storage period (at room temperature or in the environment of 2-10 degrees celsius preservation), and the supernatant can be drained and then combined to save the bottom sedimented chlorella to form a highly concentrated chlorella species;
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8. Exhaust matters in the process of algae liquid cultivation:
Friends who use transparent barrels in the second day of cultivation and later, in the shaking container or in the static state, if it is found that the algae liquid has a fine oxygen bubble that rises and produces foam on the surface of the algae liquid [normal phenomenon] caused by the container bottle, pay attention to unscrewing the container to exclude the oxygen generated; if the bag is used for cultivation, it can be adjusted appropriately according to the situation (no need for non-exhaust operation, the bag has expansion and containment);
After inoculating algae, if it is found that there are many dense oxygen bubbles attached to the wall of the barrel and the bottom, the bucket can be shaken to help the bubbles leave the barrel wall to rise, improve the permeability and light transmittance of the container, and reduce the negative impact of the oxygen bubble wall;
9. Precautions for algae liquid cultivation:
In the process of cultivation, if there is a white flocculent precipitate or floating matter at the bottom or top of the container (complex precipitation or other known or unknown cause precipitation produced by dead algae or high hardness of water), there are factors related to the water quality of each region (the reaction precipitation produced by various material components contained in the water quality of each region); if there is no impact on the proliferation of algae liquid, it can be cultivated according to the normal process; when retaining algae, please remove the sediment;
10. Prevention and control of protozoa in the cultivation process of chlorella
If protozoa appears in the breeding of chlorella and has a tendency to expand, formaldehyde can be applied to the culture medium at a concentration of 10mg/L, once a day for two consecutive days, for the prevention and control of protozoa. 1g = 1000mg, when adding, pay attention to conversion, do not overuse;
11. Dosage in chlorella production
Chlorella water body aquaculture is recommended to use 2-10 kg per mu, after the day or the next day in time to supplement the wastewater paste products, help the rapid proliferation of algae to form a dominant algae phase; in the early stage of breeding, the fertilizer water after the water disinfection in the pond can be used to splash and use amino acids per mu, if the fertilizer effect is slow, you can continue to repeat the operation once; for the water body with trace amount of moss, if you want to quickly fertilize the water, you can use it according to the amount of 10 kg per mu, and add it once according to the situation. Achieve the effect of inhibiting moss;