Recently, experts in the field of national disease control and infectious disease prevention and control published a series of special articles on the National Pathogenic Bacteria Identification Network (China Pin) in the China CDC Weekly magazine, and systematically introduced the function and mission of the National Pathogenic Bacteria Identification Network through six analysis articles and application reports. Among them, high-throughput sequencing technology plays a very important role in the functional realization of the identification network. Kan Biao, director of the Institute of Infectious Disease Prevention and Control of the Chinese Center for Disease Control and Prevention, mentioned in the opening preface of the special issue: "The gradual application of genome sequencing technology has provided massive sequence data for the detection and comparison of pathogen genes/genomes, and the era of microbial big data has arrived!" ”
Surveillance of infectious diseases can effectively prevent the introduction and transmission of pathogenic microorganisms, and early warning can effectively respond to public health emergencies, achieve early identification, early prevention and control, and provide basic guarantee for reducing morbidity and mortality. After the good development of the infectious disease surveillance system, it will enhance the ability to detect and identify infectious disease outbreaks early, and improve the sharing quality of public health information resources.
The new and recurrent emergence of infectious diseases such as the COVID-19 epidemic has put forward higher requirements for the accuracy and effectiveness of pathogenic microbial diagnosis. Although high-throughput sequencing technology (also known as next-generation sequencing technology, NGS) technology started late, is expensive, and has low popularity, it has outstanding advantages such as high throughput, high precision, and speed, and has broad application prospects in the future.
Among them, there are two main methods for identifying pathogenic microorganisms based on NGS, namely metagenomic sequencing (mNGS) and targeted NGS (tNGS).
In recent years, with the publication of more and more clinical studies and expert consensus, metagenomic next-generation sequencing (mNGS) technology has become increasingly prominent in the diagnosis of difficult and rare infectious diseases.
Compared with the wide-cast and full-capture mNGS technology, tNGS reduces the number of target pathogenic microorganisms, focuses on common clinical pathogenic microorganisms, and excludes the influence of human nucleic acid fragments on detection sensitivity through specific amplification, making NGS pathogen detection more suitable for the actual needs of relatively fixed clinical pathogen species, high sensitivity specificity requirements, and high detection cost control requirements.
In addition, nanopore sequencing technology (Nanopore) has the advantages of long length, portability, no sample amplification and real-time sequencing, which greatly reduces the detection time compared with NGS, and further meets the high requirements for detection timeliness of infectious disease pathogenic microorganisms.
The progress of sequencing technology has brought more choices for pathogenic microbial detection, but a variety of sequencing methods also mean a variety of library construction strategies, the success of library construction directly affects the sequencing quality, high requirements for the specialization of operators, need to undergo systematic training and long-term practical operation, not only time-consuming and laborious, but also have problems such as sample contamination and unstable library quality.
The EASY NGS PRE-SYSTEM automatic library preparation system was born in response to market demand, providing powerful automation solutions for mNGS pathogen detection, tNGS, Nanopore and other related library construction experiments.
EASY NGS Pre-System is a fully automatic pipetting workstation integrating pipetting module, magnetic purification module, PCR module, thermal incubation module and refrigeration module, which is suitable for library preparation of DNA and RNA samples, and can be widely adapted to the library construction process of second- and third-generation sequencing technologies such as whole genome sequencing, whole exome sequencing, targeted capture sequencing, transcriptome sequencing, metagenomic sequencing, nanopore single-molecule sequencing technology (Nanopore).
Next-generation sequencing library construction process
Third-generation sequencing library construction process
EASY NGS PRE-SYSTEM AUTOMATIC LIBRARY PREPARATION
Five features:
01
Highly automated
No manual attendance required.
Built-in code scanning and data logging system to record and track data information.
02
High safety factor
Equipped with anti-liquid dripping pipetting module, standard filter element tip, built-in high-efficiency filtration system and sterilization ultraviolet lamp, multi-angle and all-round to ensure that the whole process of experimental process is clean and pollution-free.
03
High processing efficiency
It can operate 24 hours a day, and the number of samples can be processed up to 32 at a time.
04
High accuracy
Automating standard operating procedures eliminates manual error and improves results stability and repeatability.
05
High flexibility
Library construction kits for multiple brands and sequencing types.
Customized services can be completed according to user needs.
About Nayo Biotechnology Co., Ltd
Nayolab
Focusing on life science automation, Naiyo Biotech is committed to providing customers with fully self-developed life science automation equipment and personalized solutions. Through the organic combination of R&D system and customer needs, it realizes iterative innovation of products and solutions, continuously solves pain points for customers, helps research in the field to continuously improve quality, efficiency, safety and other dimensions, and continues to promote the accessibility of automation equipment in the field of life sciences.