Rice is one of the world's most important food crops, more than half of the world's population is based on rice, pests and diseases seriously affect rice production and quality, of which brown planthopper and rice blast are the most harmful pests and diseases. The whole growth and development process of rice will continue to be damaged by brown planthoppers and rice blast, and a large number of chemical pesticides need to be applied in order to ensure high yield and stable yield, which not only causes serious environmental pollution and food safety problems, but also increases the resistance of pathogenic bacteria and plant-eating insects. The planting of resistant varieties has always been the most cost-effective and environmentally friendly strategy for preventing rice diseases and insect pests, and it is of great scientific significance for the identification and functional analysis of rice disease and insect resistance genes.
Recently, the Miao Xuexia Research Group of the Center for Excellence in Molecular Plant Sciences of the Chinese Academy of Sciences published a paper entitled "Identification of the rice genes and metabolites involved in dual resistance against brown planthopper and rice blast fungus" In the research paper, the genes and compounds with dual resistance to brown planthopper and rice blast were identified from rice varieties with high resistance to brown planthopper and rice blast by reverse genetic methods.
Transcriptome sequencing of three rice varieties with different resistances (RHT, ZH11 and MDJ) after brown planthopper and rice blast treatment, and weighted gene co-expression network analysis (WGCNA) showed that the phenylpropane pathway played an important role in defending against brown planthopper and rice blast. The expression amount of phenylpropane pathway related genes was analyzed, and five genes with the opposite trend after treatment with brown planthopper and rice blast were found, and genetic analysis found that the OsF3H gene positively regulated the resistance of rice to brown planthopper and negatively regulated the resistance of rice to rice blast. At the same time, five genes with the same trend after treatment with brown planthopper and rice blast were also found, and genetic analysis found that the knockout mutant osf3′h of the OsF3′H gene significantly improved the resistance of rice to brown planthopper and rice blast. Further metabolome analysis of three rice varieties showed that hercalyptol not only inhibited the growth of rice blast bacteria but also improved rice resistance to brown planthoppers.
The results of this study show that the phenylpropane pathway plays a very important role in the process of rice resistance to brown planthoppers and rice blast, and is a resistance metabolic pathway worthy of in-depth study.
Original link:
https://onlinelibrary.wiley.com/doi/10.1111/pce.14321
Source: Plants and the Environment PCE
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