<h2 id="hi-260" > "National Compilation of Chinese Herbal Medicine": Xianhe Grass</h2>
Pinyin Xiān Hè Cǎo
The English name is HERBA AGRIMONIAE
Also known as Dragon Bud Grass, Detachment Grass, Wolf's Tooth Grass, Golden-crowned Dragon's Tooth, Yellow Dragon's Tail, and Hairy Foot Yin
The source of this product is the Rosaceae plant Agrimonia pilosa Ledeb. The dried aerial part. In summer and autumn, when the stems and leaves are luxuriant, they are cut, impurities are removed, and dried.
Traits This product is 50 ~ 100 cm long, the whole is covered with white soft hairs, the lower part of the stem is cylindrical, the diameter is 4 to 6 mm, reddish brown, the upper part is square cylindrical, the four sides are slightly concave, green-brown, there are longitudinal grooves and ridges, there are nodes; the body is light, hard, easy to break, and the section is hollow. Singular pinnate compound leaves are alternate, dark green, wrinkled and curled; brittle and brittle; the leaves are 2 kinds of large and small, interspersed on the leaf axis, the apical leaflets are larger, the intact leaflets are flattened and ovate or oblong-oval, the apex is pointed, the base is wedge-shaped, and the edges are serrated; the leaves are 2, the stem is held, and the obliquely ovate. The inflorescence is slender, the lower part of the calyx is cylindrical, the upper part of the calyx has barbs, the apex is 5 lobed, and the petals are yellow. The smell is slight, the taste is slightly bitter.
Identify the powder dark green of the leaves of this product. Upper epidermal cells are polygonal; the epidermal cell wall is wavy curved, stomatal infinitive or inequality. Non-glandular hairy single cell, varying in length, thick walls, woody, warty-like protrusions, rarely spiraly. The small glandular hairs have 1 to 4 cells on the head, oval in shape, and 1 to 2 cells in the stalk; there are a few glandular scales, single cells on the head, about 68 μm in diameter, containing oil droplets, and single cells in the stalk. Calcium oxalate clusters are numerous, with a diameter of 9 to 50 μm.
Process to remove residues and impurities, wash, moisten slightly, cut into sections, dry.
The sexual taste is bitter, astringent and flat.
Return to the heart and liver meridians.
Functional indications for astringent hemostasis, malaria amputation, diarrhea, detoxification. It is used to cough up blood, vomit blood, collapse and leakage of blood, malaria, blood diarrhea, deflection strain, carbuncle sore poison, itchy band under the vagina.
Dosage 6 to 12 g. Apply appropriate amount for external use.
Store in a ventilated, dry place.
Excerpt from the National Compilation of Chinese Herbal Medicine
<h2 id="hi-10945" > the Dictionary of Chinese Medicine: Crane Grass</h2>
Aliases Dragon's Tooth Grass, Shizhou Dragon's Tooth Grass ("Bencao Tujing"), Melon Vanilla ("Saving the Wilderness Materia Medica"), Huanglongwei ("Yunnan Materia Medica"), Iron Wasp, Golden-topped Dragon Bud ("Hundred Grass Mirror"), Old Stork's Mouth, Motherwort, Mao's Foot Yin ("Botanical Names") ("Botanical Names"), Huanglongya, Grass Dragon's Tooth, Ground Pepper, Yellow Flower Grass, Snake Knot (Henry's "Chinese Botanical List"), Dragon Head Grass, Inch Eight Sections ("Classification of Herbs"), Passing Yellow, Hairy Foot Chicken ("Tianbao Materia Medica"), Jerry Flower, Thread Hemp Flower ("Botanical Collection"), Deli Grass (Atlas of Southern Yunnan Materia Medica), Knife Edge Medicine, Da Mao Medicine (Guizhou Folk Medicine Collection), DiXian Herb (Northeast Medicine Plant"), Snake Regression ("Southern Yunnan Materia Medica"), Roadside Chicken, General Mao, Chicken Claw Sand, Roadside Yellow, Five-Hoof Wind, Oxal Grass (Hunan
Source :Identification of Counterfeit Medicines
The source is the whole grass of the rosaceae plant Longaer. In summer and autumn, when the branches and leaves are luxuriant and not flowering, cut the whole grass, remove the soil, and dry it in the sun.
Original Form Dragon Bud Grass ("Saving the Wilderness Materia Medica")
It is a perennial herb growing 50 to 120 cm tall. The stem is erect and covered entirely with long white soft hairs, sometimes scattered with short soft hairs, and the upper part of the branches. Singular pinnate compound leaves, alternate, with stalks: 2 leaves, obliquely ovate, with deeply lobed teeth, covered with long soft hairs; small leaves 3 to 9, oblong or oval, 1 to 6 cm long, 0.6 to 3 cm wide, sharply pointed at the apex, wedge-shaped at the tomb, sometimes slightly oblique, sharply serrated at the edges, soft hairs on both sides, with most yellow glandular spots; the apical and central leaves are larger, with a few pairs of small leaves in between. The inflorescence is apical and axillary, narrow and slender, 10 to 20 cm long; the flowers have short stems and 2 trigeminal bracts at the base; the calyx is simple, the apex is 5 lobes, the lobes are inverted ovate, densely hooked; the petals are 5, yellow, inverted ovate, the apex is slightly concave; the stamens are 10 or more; the peduncles are 2, and the stigma is head-like. Skinny fruit, wrapped in a hooked calyx. Flowering period is from July to September. The fruiting period is from September to October.
The roots of this plant (dragon's tooth grass root) and the rhizome with adventitious buds (crane grass root buds) are used for medicinal purposes, and each is detailed.
Habitat segments grow in wastelands, hillsides, roadsides, and meadows. It is distributed in most parts of our country. The main products are Zhejiang, Jiangsu and Hubei. In addition, Anhui, Fujian, Guangdong, Hebei, Shandong, Hunan, Yunnan and other places are also produced.
Dry whole grass, lignified at the base of the stem, light brown to purple-red, 4 to 6 mm in diameter, smooth and hairless, the stem nodes are obvious, the upper and lower are dense, sometimes there are residual leaves; the upper stem is greenish brown, or pale yellowish brown, covered with white soft hairs, the leaves are gray-green, wrinkled and curled. Occasionally, flower or fruit branches are seen. The breath is slight, the taste is slightly bitter. It is preferable to have purple-red stems, tender branches, and complete leaves.
The chemical composition of the whole herb contains xianhe oxalis, xianhe lactone, tannin (caramelized catechin tannin, gall tannin, etc.), sterols, organic acids, phenolic components, saponins and so on. The roots contain 8.9% tannin, the stem contains tannin 6.5%, and the leaves contain tannin 16.4%. The stems and leaves also contain hyolin-7-β-glucoside and apigenin-7-β-glucoside.
Concoct to remove impurity residues, wash, moisten, cut off, and dry in the sun.
Sexual taste bitter and hard, flat.
(1) "Lu Wei Yan Materia Medica": "The taste is spicy, warm, non-toxic." "
(2) "Yunnan Materia Medica": "The sex is slightly warm, and the taste is bitter." "
(3) "Raw Herbal Sexual Preparation": "Sweet taste, flat sex." "
It is incorporated into the lungs, liver, and spleen meridians.
Function indications for hemostasis, stomach health. Treatment of hemoptysis, vomiting blood, blood in urine, blood in the stool, red diarrhea, collapse under the band, strain loss, carbuncle swelling, bruises, trauma bleeding.
(1) "Lu Wei Yan Materia Medica": "Leaf: Cure sore ringworm." "
(2) "Yunnan Materia Medica": "Cure the woman's menstruation or before or after, under the red white belt, cold and abdominal pain in the face, and long-term red and white blood diarrhea." "
(3) "Raw Herbal Sexual Preparation": "Rationalize bruises, stop bleeding, and scatter sores and poison." "
(4) "Hundred Grass Mirror": "Under the qi to activate the blood, rationalize the hundred diseases, scatter the full; fall and vomit blood, blood collapse, diarrhea, intestinal wind under the blood." "
(5) "Botanical Names and Facts Tu Kao": "Cure wind phlegm and low back pain." "
(6) "Pseudo-medicine identification": "Cure fistula." "
(7) "Modern Practical Chinese Medicine": "It is a strong astringent hemostatic agent, and has a cardiotonic effect." It is suitable for hemoptysis, intestinal bleeding, gastric ulcer bleeding, uterine bleeding, dental bleeding, hemorrhoidal hemorrhoids, liver abscess and other symptoms. "
Dosage for internal use: decoction, 3 to 5 yuan (fresh 0.5 to 1 two), mashing juice or into powder. External use: mashing.
Compound (1) treatment of pulmonary tuberculosis hemoptysis: fresh crane grass one or two (dried, six dollars), sugar one or two. Mash the crane grass, add cold boiling water, bowl, stir, squeeze the juice, add sugar and take it once. (Guizhou Folk Medicine Collection)
(2) Cure vomiting blood: crane grass, deer horn grass, wheat bottle grass. Boil water to serve. (Journal of Sichuan Traditional Chinese Medicine)
(3) Treatment of nosebleeds and blood in the stool: crane grass, pu huang, thatch root, thistle. Fry. (Journal of Sichuan Traditional Chinese Medicine)
(4) Cure also diarrhea and hemoptysis, vomiting blood: dragon bud grass three to six dollars, water frying. (Lingnan Medicine Collection)
(5) Treat women's menstruation or before or after menstruation, sometimes low back pain, fever. Flatulence: Huanglongwei two dollars, Hangzhou peony three dollars, Sichuan root one dollar five points, incense with one dollar, safflower two points, fried, order wine. Such as menstrual blood purple black, plus sumac, skullcap; abdominal pain plus yan husso, cumin. (Southern Yunnan Materia Medica)
(6) Cure the red white belt or white turbidity: three dollars for the yellow dragon tail, one dollar for the root of the horse whip, and two dollars for the black lock Megan. Order water and wine to serve. (Southern Yunnan Materia Medica)
(7) Treatment of anemia and weakness, energy impotence (folk treatment of fatigue): one or two crane grasses, ten red dates. Fry in water and serve several times a day. (Modern Practical Chinese Medicine)
(8) Cure children's diarrhea summer: crane grass five dollars, red dates seven grains, water frying. (Zhejiang Tianmu Yam PlantAtion)
(9) Cure children's chancre: dragon bud grass five to seven dollars, remove the root and stem on the coarse skin, combine the pork liver three to four two, add water and cook until the liver is cooked, remove the residue, drink soup and eat liver. ("Jiangxi Folk Herbal Medicine Prescription")
(10) Cure malaria, daily attacks, chest and abdominal fullness: crane grass three dollars, ground into a fine powder, swallowed with shochu before malaria, with three doses. (Guizhou Folk Medicine Collection)
(11) Cure allergic purpura: three or two crane grass, one or two raw turtle versions, two or two goji root and ground elm charcoal. Serve fried in water. (Su Yi's Handbook of Chinese Herbal Medicine)
(12) Cure carbuncle poisoning: fresh dragon bud grass four two, sweet potato wine half a catty, rinse water, stew, take after meals. The first person to take three or four doses can be dissolved, if it has become pus, even take more than ten doses, can be anti-inflammatory and pain-relieving. (Mindong Materia Medica)
(13) Cure cradle carbuncle, the initial rise of the elimination, the pus of the ulcer, and can make the pus out of not much: dragon bud grass one or two, half a pot of white wine, fried to half a bowl, full after serving. ("Hundred Grass Mirror")
(14) Treatment of redness and swelling from falls: crane grass, small blood vine, white flower grass (stir-fried in wine, those who break the skin of trauma do not use wine to fry). Mash the flannel and take it inside with soaked wine. (Journal of Sichuan Traditional Chinese Medicine)
(15) Cure snake bites: fresh dragon bud grass leaves, wash, mash the wound. (Fujian Folk Herbal Medicine)
Clinical application (1) for hemostasis
Crane grass and the crane grass extracted from the stem and leaves of the crane grass, its hemostatic effect has been clinically confirmed, and has long been made into various dosage forms widely used in various bleeding disorders. In recent years, crane grass has been made into hemostatic powder for traumatic bleeding, bleeding or bleeding during visceral surgery (including intracranial surgery, chest and abdomen surgery), according to the clinical observation of 20 cases, all of which stopped bleeding within 1 to 2 minutes.
(2) Treatment of trichomoniasis vaginitis
Using the stems and leaves of wolf's tooth grass, make a 200% concentrated decoction. When used, the vagina is first dilated with a vaginal speculum, and the vaginal wall is thoroughly and thoroughly washed with a gell cotton ball, and then the cotton ball full of wolf's tooth grass liquid is evenly applied to the entire vaginal wall. Then stuff a special large cotton plug with a full of wolf's tooth grass liquid, leave it for 3 to 4 hours, and instruct the patient to remove it by himself. Once a day, 7 times a course of treatment. According to the observation of 40 cases, the cure rate of 3 courses reached 92.5%, and the microscopic examination of Trichomoniasis vaginalis was negative three times; the rest of the cases also improved. Generally, after about 1 week of medication, local itching is relieved or disappeared, and vaginal discharge is significantly reduced.
(3) Treatment of infectious food poisoning of salt-loving bacteria
Crane grass 1 two, fry into 100 ml, 1 time to serve. At the same time, it is combined with rehydration to correct dehydration, and atropine is used to rescue shock and stop spasticity. After the above treatment, the general symptoms of poisoning in 108 patients included chills, vomiting, headache, chest tightness, limb numbness, etc. were eliminated within 2 to 3 hours, abdominal pain disappeared in about 12 hours, and diarrhea was generally controlled within 24 to 48 hours.
(4) Rescue complete AV block caused by Keshan disease
After the diagnosis is confirmed, it is directly injected intravenously with 25 to 50% glucose 20 to 40 ml with 5 to 10 mg of xianhelin, and can be repeated every 3 to 4 hours if necessary. The injection must be slow and the injection time shall not be less than 10 minutes. In the clinical trial, 6 patients and 4 patients were converted to sinus rhythm after treatment, and the clinical symptoms also improved rapidly; 2 cases were ineffective. It is believed that xianhelin mainly works by relieving vagus nerve inhibition and increasing the heart rhythm; the repair of myocardial damage and the improvement of myocardial metabolism are not very obvious.
(5) Others
Take 0.5 to 1 decoction of crane grass, 1 dose per day, for 5 to 7 days, which has a certain preventive effect on leptospirosis of influenza typhoid fever.
Remarks In addition, there are also the same genus of plants blunt-toothed dragon bud grass (northeast), fluffy dragon bud grass, sparse haired dragon bud grass, Korean dragon bud grass (northeast), multi-toothed dragon bud grass (northeast Daxinganling), Asian dragon bud grass (also known as Xinjiang dragon bud grass) and so on are also the same human medicine.
Excerpt from the Dictionary of Chinese Medicine
<h2 id="hi-29046" > Chinese Materia Medica: Crane Grass</h2>
Herb of Hairyvein Agrimonia Herband Bud, Herb of Hairyvein Agrimonia
Alias crane grass buds, dragon's tooth grass, Shizhou dragon's tooth grass, melon vanilla, yellow dragon's tail, iron wasp, golden-topped dragon bud, old stork's mouth, motherwort, hairy foot, yellow dragon's tooth, grass dragon's tooth, ground pepper, yellow flower grass, snake knot, dragon head grass, inch eight knots, passing yellow, hairy foot chicken, Jerry flower, nettle flower, detachment grass, knife mouth medicine, big hair medicine, earth immortal grass, snake regression, roadside chicken, general Mao, chicken claw sand, roadside yellow, five-hoofed wind, minotaur grass, diarrhea grass, yellow flower boy, heterophylla, subordination mother, paternal grass, hairy chicken grass, group orchid septic herb, Wolf's tooth grass, hemostatic grass yellow dragon tooth.
The source is from 1. "Identification of Counterfeit Medicines".
2. "Medicine Mirror • Picking Up The Gift": Phlegm of rolling throat diaphragm, flattening of the stomach, stone piercing who knows who. Note: Choking diaphragm turning the stomach, from the doctor to the sick, the group is afraid, thinking that the incurable disease, yu get this agent, ten to nine effects. It is a song to be made. Song Yue: Who knows the stone piercing, green leaves with deep serrated edges, wide and not inch long and more times longer, round stems and branches are held up and connected, autumn hair yellow flowers fine petals five, strong flat small needle thorns, su root three feet of xu, seeds and spring seedlings with brother shoulders, large leaves sandwiched in the middle of small leaves, layers of contrast fresh, taste bitter and hard into the lungs, through the intestines through the stomach can be attacked, picking stems and leaves with mash juice, cane syrup liquor
Source medicinal base source: the aerial part of the Rosaceae plant Dragon Bud Grass.
Latin botanical animal mineral name: Agrimonia pilosa Ledeb.
Harvesting and storage: In summer and autumn, when the branches and leaves are luxuriant and not flowering, cut the whole grass, remove the soil, and dry it in the sun.
Protoform Dragon Bud Grass Perennial grass, 30-120 cm tall. The rhizomes are short and often have 1 or more underground buds at the base. The stem is sparsely hairy and short, and the sparse lower part is sparsely haired with stiff hairs. Odd pinnate compound leaves alternate; leaf sickle-shaped, sparsely ovate, sharply pointed or tapering at the apex, sharp serrations or lobes at the edges, sparse margins; leaflets have 2 kinds of sizes, interspersed on the leaf axis, larger leaflets 3-4 pairs, rare 2 pairs, upward reduction to 3 leaflets, leaflets are almost sessile, inverted ovate to inverted ovate lanceolate, 1.5–5 cm long, 1–2.5 cm wide, sharply pointed to rounded blunt at the apex, sparsely pointed, wedge-shaped at the base, sharply pointed to round blunt serrations at the edges, green above, sparsely hairy, pale green below, sparsely hairy on the veins, sparsely hairless, with significant glandular spots. The inflorescences are single or 2-3 born at the top of the stem, the inflorescence axis is softly hairy, the peduncle is 1-5 mm long, covered with soft hairs; the bracts are usually 3 deeply lobed, lobed bands, small bracts are opposite, ovate, full margin or marginal; the flower diameter is 6-9 mm, sepals 5, triangular ovate; petals 5, oblong, yellow; stamens 5-15; peduncle 2, filamentous, stigma-headed. The fruit is inverted oval-conical, with 10 ribs on the outside, sparsely hairy, with several layers of barbs at the apex, upright at the young, leaning inwards when ripe, with 7-8 mm long barbs and about 3–4 mm in diameter at the widest point. The flowering period is from May to December.
Habitat division ecological environment: grows in streams, roadsides, grasslands, shrublands, forest edges and under thinning forests.
Resource distribution: It is distributed in most parts of China.
Cultivation techniques Propagation with seeds or roots. Seed propagation: spring sowing in the south in late March, north in mid-April and late April, autumn sowing, southern in late September to early October, and the north before freezing in late October to early November. Make a 1.3 m flat furrow on the prepared ground, sow a ditch 1-2 cm deep according to the row spacing of 30-40 cm, spread the seeds evenly into the ditch, cover the thin soil, slightly press and water. The sowing amount per 1hm\2 is 15-22.5kg, the germination temperature is 25 °C, and the seedlings emerge 10-15 days after sowing. Root propagation: Spring and autumn can be carried out, the roots are dug out and split, each with 2-3 root buds, planted in time. The hole planting is 30 cm × 15 cm according to the row spacing, digging 15 cm deep holes, 1 hole per hole, covering 5 cm, compacting and watering. If it has sprouted, the buds will be exposed to the ground when planting, and watered after planting, and the seedling emergence rate can reach more than 95%.
Field management Seedling height 3-5 cm time seedlings, supplement seedlings, plucked dense weak seedlings, seedling height of 15 cm according to the plant spacing of 15 cm seedlings. Combined with loose soil to hoe grass, the seedlings are no longer loose after sealing, and the grass is pulled out in time. In order to increase the yield of nitrogen fertilizer at the seedling setting stage, human feces and urine per 1 hm \ 2 1500-22500 kg, appropriately increase the application of phosphorus and potassium fertilizer to promote root growth.
Trait Identification The whole is 50-100 cm long and covered with white soft hairs. The lower part of the stem is cylindrical, 0.4-0 .6cm in diameter, reddish brown, the upper part is square-cylindrical, slightly concave on all sides, green-brown hairs, longitudinal grooves and ridges, nodes, light, hard, easy to break, hollow section. Singular pinnate compound leaves alternate, dark green, wrinkled and curly; brittle, brittle, brittle; leaves are 2 kinds of sizes, alternately grow on the leaf axis, the apical leaflets are larger, the intact leaflets are ovate or oblong-oval after unfolding, the apex is pointed, the base is wedge-shaped, and the edges are serrated; the leaves are 2, holding the stem, obliquely ovate. The inflorescence is elongated; the flower diameter is 0.6-0.9 cm, the lower part of the calyx is cylindrical, the upper part of the calyx has barbs, the apex is 5 lobes, and the petals are yellow. The fruit is 0.7–0.8cm long and 0.3–0.4 cm in diameter. The smell is slight, the taste is slightly bitter. It is preferable to be tender and have many leaves.
Microscopic identification Powder characteristics: dark green. (1) Upper epidermal cells polygonal; epidermal cell wall wavy curved, stomatal infinitive or inequality. (2) Non-glandular hairy single cell, different length, wall thickness, woodification, warty protrusion, a few spiral texture. (3) Glandular hair head 1-4 cells, oval, stalk generally 1-2 cells, shorter; there are a few glandular scales, head single cells, about 68 μm in diameter, containing volatile oil droplets, stalk single cell. (4) Calcium oxalate cluster crystals are very large, with a diameter of 9-50 μm.
Chemical ingredients whole grass contains xianhe oxal, xianheolide (Agrimonolide), tannin (for coke cokecapol tannin, gallic tannin, etc. (6.67%-13.71%)), sterols, organic acids, phenolic components, saponins, and also isolated xianhe phenol A (agrimol A), xianhe phenol B (agrimol B), xianhe phenol C (agrimol C), xianhe phenol D (agrimol D), xianhe phenol E (agrimol E), Crane phenol F (agrimol F) and crane grass phenol G (agrimol G), of which the structure of crane grass phenol C has been clarified; it still contains luteolin-7-D-glucoside, cosmosin (cosmosin) and volatile oils, etc., and also isolated from the whole grass A, B, C (agrimonin A, B, C), commercially available crane herb as an acidic substance, medicinal sodium salt, may be a mixture. The roots contain 8.9% tannin, the stem contains tannin 6.5%, and the leaves contain tannin 16.4%. The stems and leaves also contain luteolin-7-β-glucoside (Luteolin-7-β-glucoside) and apigenin-7-β-glucoside (Apigenin-7-β-glucoside) and vitamins C and K. Fresh rhizome winter bud contains crane phenol (agrimophol), which has been proved to be an effective substance by in vitro cystsitr tests and clinical observations, and also contains agrimonolide(agrimonolide), vanillic acid (vamillic acid), l-flagsinin (l-dihydroquercetin, l-taxifolin), laminic acid, pseudo-herbicin, cysperm lactone-6-O-β-D-glucoside, trans-parahydroxycinnamate, (2S,3S)-(-)- Ginzapinin-3-O-β-D-glucoside, 4-O-β-D-xylonoside, etc.
Pharmacological effect 1.Insecticidal effect: 1.1.Repellent effect on schistosomiasis: 1.1.1.Effect of crane phenol on the activity of culturing schistosomiasis in vitro: From rabbits infected with 1000-1500 Japanese schistosomiasis tail larvae for 5-6 weeks, insects are taken by sterility and cultured in vitro according to conventional methods. Pick a complete pair of co-hugging male and female worms and place them in a Carnota bottle containing 1 ml of calf serum-Tai's solution (1:5), 10 bottles per group, and culture in an incubator at 36-37 °C. After the insect body is normally active, add the desired concentration of the hydrangea phenol solution with a capacity of 1 ml, and observe the insect body activity under the dissecting mirror on time. Each trial was controlled with a culture medium containing arginine, Tween 80 and polyethylene glycol 400. The experimental results showed that the amphoteric schistosomiasis cultured in vitro was exposed to crane phenol for about 4 μg/ml for about 4 minutes, that is, the insect body was excited and showed rapid peristalsis; after 6 minutes, the abdominal suction cup fell off from the bottle wall, but it could be sucked repeatedly, and the suction ability was completely lost after about 2-3 minutes. At this time, only the abdominal suction cups still have a slight contraction, the worm's body part is gradually contracture, about 20-30 minutes the male worm stops moving, but the female worm's suction cup is still slightly constricted in l minutes. The concentration of the solution is 8 μg/ml, and the male worm stops moving after 6-15 minutes of contact with the solution, and the female worm stops moving within 2 hours of contact with the solution. The addition of atropine (1×10(-3)M) or serotonin (1×10(-3)M) after amphoteric schistosomiasis has ceased to be active does not restore the worm's activity. Normal amphoteric schistosomiasis exposed to betel nut (1×10 (-3)M) immediately relaxes and elongates, the female grooves spread out, and the worms stop moving. At this time, 1 min after the addition of 8 μg /ml of crane phenol, the insect body begins to resume activity and gradually excites, lasting about 2 minutes, the insect body contracts, and the activity gradually weakens until it stops activity again. When amphoteric schistosomiasis was cultured with a culture medium containing crane hydroxysophene 0.5-2 μg/ml, if the culture solution was not exchanged, the time of the male insect activity stopped was 2.8±0.4 and 2.9±0.3 days (X±SE, the same below), the female was 5.2±0.4 days; if the concentration of the liquid was 4 μg/mL, the time of the male insect activity stopped was less than 30 minutes, and the female insects were 1.4±0.2 and 1.6±0.2 days, respectively; when the concentration of the liquid was 8 μg/ml, The male worm stops moving within 15 minutes and the female stops moving within 2 hours. After 24, 48, 72 and 96 hours of contact with 8 μg/ml of amphoteric schistosomiasis and crane phenol, respectively, the first group of worms was transferred to normal culture solution, and the worms could gradually resume weak activity but died within 3 days, while the last three groups did not resume activity. The average survival time of amphoteric schistosomiasis in the control group was 12.1±0.6 days.
1.1.2. Effect of crane phenol combined with nizolidium on the cultivation of insects in vitro in infected mice: 80-100 mice infected with schistosomids tailworm for 5 weeks, 300 mg/kg, nizolidium 75 mg/kg or two drugs combined with gastric lavage treatment, the course of treatment is 5 days. The administered mice were dissected 24 hours after discontinuation, and the amphoteric schistosomiasis was collected from the mesenteric membrane and liver for in vitro culture to observe the activity and recovery of the insects. The experimental results showed that the amphoteric schistosomiasis in the group of crane hydroxol and nirazolidium could not return to normal activities and died within 3 days. The mean survival time of insects treated with crane hydroxol or nirazolidium alone was 12.1±0.5 and 13.3±0.7 days, respectively, which was very significantly different from the combined treatment group (P<0.01).
1.1.3. Effect of crane phenol combined with nirazolidium on the survival time of schistosomids: in a group of 10 pairs of insects, the average survival time of male insects was 1.5±0.5 days, and the average survival time of female insects was 2.4±0.2 days in a group of 10 pairs of insects. In the culture medium containing nizolidium 10 μg/ml or crane phenol 0.25 μg/ml, the average survival time of male insects was 3.3±0.2 and 3.1±0.2 days, and the female insects were 4.4±0.4 and 5.4±0.4 days, respectively, accounting for a very significant difference in the combined group (P<0.01), and the results of the secondary experiments were similar. It is also reported that crane phenol also has a strong killing effect on blood suckers in bovine and sheep liver tablets, and has a good therapeutic effect on bovine and sheep liver hemoematodies. Crane phenol alone for mouse schistosomiasis effect is not good, when combined with nizolidium, it significantly improves the treatment effect, the female rate of sick dogs can be reduced by up to 98-100% In addition, the glycogen content of the worm body decreases significantly after taking crane phenol, but the recovery is fast, and the combined administration is similar to the above.
1.2. Killing effect on tapeworms and roundworms: 1.2.1. Effect on pig cysticercosis: After slaughtering a sick pig suffering from pork tapeworm cysticercosis, peel off the cysticer cysticercosis, put it into the liquid prepared with nutrient solution, soak it for l hours at room temperature (16-16.5 °C), and shake it 3-5 times at intervals, then take out the nutrient solution and wash it 2-3 times, put it into the 80% bile saline physiological water at a constant temperature of 38 °C, observe the percentage of incubation of the worms within 1 hour, and compare the effects of each concentration of the drug. Stripped 286 porcupine cysticercosis, divided into 7 groups, each group of 38-49, respectively, put in 5×1O (-4), 2×10 (-4), 10 (-4), 10 (-5), 10 (-6) and 10 (-7) crane phenol liquid, according to the above method of immersion, warm incubation culture, the result of nutrient solution control group in l hour the incubation rate of live insects 87.8%, 10 (-7) zygosate phenol has no significant effect, the incubation rate of 36.7%, with the increase of drug concentration, the larval hatching rate gradually decreased. Concentrations of 5×10(14) completely inhibit the hatching of larvae.
1.2.2. Effect on the effect of pig tapeworm and the effect of different pulverization degrees of the drug: according to the above grinding and dispensing method, prepare 10 (-4) crane phenol solution, warm to 38 ° C; over 200 mesh, 160-100 mesh and 50 mesh sieve powder weigh 10mg each, add 20% acacia solution 10ml at the same speed, the same number of times stirred and mixed, with a coarse mouth pipette each suction 2ml into a warm to 38 ° C 18ml nutrient solution, Mix well to form a concentration of 10 (-4). The pig tapeworms hatched according to the above method are placed in the solution of each drug just prepared and incubated in a 38 ° C water bath. Taking the complete cessation of insect activity as an indicator, the average time and time range of the inactivity of each group of tapeworms after being put into the liquid were recorded, and the average time and time range of the small tapeworms stopped in activity were calculated. According to the above method, the cysticercosis hatched out of the juvenile tapeworm, 100 well-moved people, divided into 5 groups, 20 in each group, respectively, into the newly prepared drug preparations of different crushing degrees, for warm incubation observation. As a result, the 10 (-4) liquid prepared by grinding method completely stopped inactivity 5-30 minutes after contact with the pig tapeworm, the average stop-activity time was 15.5 minutes, and the larvae were removed and put fresh nutrient solution still did not recover, which showed that the concentration of crane phenol could completely kill the pig tapeworm. The 10 (-4) powder suspension over 200 mesh, 160-100 mesh and 50 mesh sieves completely stops the worm in the time range of 5-50, 10-50, and 30-60 minutes, respectively. The finer the pulverization, the faster the drug works, from grinding the formulated solution to the powder suspension of the 50 mesh sieve, so that the average time for the worm to stop activity is almost in a straight line. The larvae of the control group are all active within 90 minutes of observation.
1.2.3. Effect on Taenia Mohs: Take the extended Moniz tapeworm (Monieziaexpensa, hereinafter referred to as Mohs taeniasis) of fresh sheep and put it into a nutrient solution at 38 °C for 15 minutes, and stimulate the neck chain and body segments near the head with a Hagad's inductive stimulator (current 0.3-0.4 mA) (including writing ovules), select those with good spontaneous activity and normal local and systemic reactions after electrical stimulation for experimental purposes. Suitable tapeworms were placed in different concentrations of medicinal solutions at 38 °C constant temperature culture, and the spontaneous activity of insects and responses to electrical stimulation were observed at 1, 2, 5, 20, 40, 60 minutes and other times. Remove after 1 min, rinse with nutrient solution and place into fresh nutrient solution at 38 °C to observe spontaneous activity and response to electrical stimulation. The effect of the drug was observed in those who died after spontaneous activity stopped and there was no local and systemic response of the worm after electrical stimulation. 32 Tapeworm Moses were selected and divided into 6 groups, and the experiment was carried out in stages. The insects were placed into 10(-4), 10(-5), 2×10 (-6), 10(-6) solution of crane grass phenol and 0.69×10 (-5) of antipyrospyrifos (the same molecular concentration of grams as 10 (-5) crane phenol, both 2.1×10 (-4) grams of molecules) solution and nutrient solution (control) and the nutrient solution (control) was incubated and observed according to the above method, and the results showed that the spontaneous activity of the above four concentrations of crane phenol solution was enhanced within 1 minute after contact with the insect body, and the insect body began to contract. Spontaneous activity stopped for 2 minutes, the worm body became narrow and thickened, the edges were jagged, contracture, and the response to electrical stimulation was positive. At 5 minutes, the extreme contracture of the worm body is spirochetes, and the response to electrical stimulation is locally or whole-body, and some of the post-ovulation fragments of the worm body are shed. The degree of contracture decreases slightly after about 20 minutes, and the body cannot fully recover the contracture state after being pulled. After 60 minutes of maceration, tapeworms in the 10 (-6) concentration group resumed spontaneous activity, and some of the insects in the 2× l0 (-6) concentration group resumed spontaneous activity or responded positively to electrical stimulation, while the 10(-4) and 10(-5) concentration groups did not recover.
1.2.4. Effect on short-membrane-shell tapeworm disease mice: Experimental treatment of 92 mice artificially infected with Taenia brachycephalus, randomly divided into 8 groups on the 14th-21st day after infection, respectively, 1 gastric crane phenol 0.1-0.2g/kg, petroleum ether extract 0.6-0.8g/kg, acid precipitate 0.4g/kg, antipyridoxine 0.3g/kg, dedine 0.5g/kg and normal saline 20ml/kg, respectively, executed on the 3rd day after administration, and the entire intestinal tube was removed for examination according to the above method. The results showed that the deflection rate of 0.1 g/kg of crane hydroxysophene was 66.7%, and when the dose was 0.2 g/kg, all the intestinal short-membrane taeniae in artificially infected mice could be expelled. Petroleum ether extracts, acid precipitates and two preparations also have a depressive effect. The principle of crane phenol insecticidal tapeworm may be caused by it significantly and persistently inhibiting the metabolism of insect body cells, cutting off the energy supply that sustains life.
1.2.5. The ex vivo tapeworm cysts are placed in various sub-concentrations of crane grass rhizome winter bud extract, 39 °C for 2 hours, 0.125mg/kg concentration when the cysticer kill rate is 50%; under the microscope, the extract is added in drops on the larvae, the suction cup on the head segment can be seen, the apical movement is quickly inhibited, the head segment is solid and stiff in 4-7 minutes, the suction cup and the apical node are completely withdrawn, and its active ingredient mainly acts on the cephalic segment, which also has an effect on the cervical segment and the body segment. The active ingredient of crane grass is crane grass phenol, which is based on the principle of action of inhibiting the metabolism of insect cells, cutting off the ability of insects to maintain life, and making the insects shrink strongly, increasing energy consumption, and accelerating the death of insects.
1.2.6. Effect on ex vivo pig roundworms: Take 20 newly slaughtered pig roundworms (both male and female), divide them into four groups, and put in nutrient solution and 10 (-5), 10 (-4), 10 (-3) crane phenol solution at 38 °C. Roundworms have an excitatory effect within 5 minutes after immersion in 10 (-5)-10(-3) liquid, 10-30 minutes the strongest effect, the body expansion and contraction is extremely intense, entangled with each other or the insect body stands up and protrudes out of the cup, at this time the insect body has a certain degree of spasm, after 40 minutes the activity is weakened, after 60 minutes the roundworm in the immersion group is still more active than the control group. It can be seen that crane phenol has a long-lasting excitatory effect on roundworms.
2. Spermicidal effect: 2.1. Effect of xianhe grass root bud petroleum ether extract on animal sperm: experiments were carried out with the fu testicles of 6 mice and 4 guinea pigs, the sperm of the two animals were each subjected to two concentrations of petroleum ether extract, the mouse sperm was each experimented 5 times, and the guinea pig sperm was each experimented 3 times. ResultsThe suspension of the petroleum ether extract containing crane hydrangea 3.1×10(-4) and 6.2×10(-4) g/ml concentrations contained crane oxenol, all of which killed all mouse sperm within 5 minutes. Petroleum ether extract 3.1×10(-4)g/ml can also kill all guinea pig sperm in 5 minutes, while the concentration doubles, 6.2×10 (-4)g/ml kills all guinea pig sperm in 1 minute.
2.2. Effect of xianhe grass petroleum ether extract on human sperm: the semen of two people is used for testing, some tests are used to dilute semen, and some tests are carried out with raw semen, and two tests are carried out each. Results The suspension of petroleum ether extract containing 5×10(-4)g/ml concentration of human diluted semen can kill all sperm within 3-7 minutes, and the concentration of 2.5×10 (-3)g/ml containing crane phenol can kill all sperm in 1-5 minutes.
2.3. Effect of crane phenol suspension on animal sperm: 1 part of crane phenol crystal dissolved in 10 parts of chloroform, added in 50 parts of polyethylene glycol (PEG300) in stages, studied well, and then added 5% arabic gum prepared with Ren's liquid, while adding research, formulated into a suspension of the required concentration. The preparation of the control solution, except for the absence of crane phenol, other components and preparation methods are the same as the crane phenol suspension. Different concentrations of crane phenol suspension and control solution were diluted with Ren's solution. The test was carried out with the fu testicles of 5 mice, 2 guinea pigs and 2 rabbits, and the sperm of the three animals were tested with 2-3 concentrations of the crane phenol suspension, and various concentrations were tested 2-4 times respectively. Concentrations of 1×10(-4) and 2×10(-4)g/ml of crane phenol suspension can kill all sperm of mice, guinea pigs and rabbits within 5 min, and 3×10 (-4) and 5×10 (-4) g/ml can kill 3 animal sperm in 1 min.
2.4. Effect of crane phenol suspension on human sperm: the semen of 3 people was used for testing, part of the test was used to dilute semen, and part of the test was used with raw semen. There are 4 concentrations of crane phenol suspension for dilution semen test, 2 concentrations of crane phenol suspension for the original semen test, and 3-5 tests are carried out at various concentrations. The 2×10(-4) and 3×10(-4) g/ml concentrations of the 2×10(-4)g/ml concentration of human diluted semen can kill all sperm within 5 minutes, 4×10 (-4) and 5×10 (-4) g/ml can kill all sperm in 1 minute, while the original semen of humans requires higher concentrations, 1×10 (-3)g/ml can kill all sperm in 5 minutes, and 2×10(-3)g/ml can kill all sperm in 1 minute.
3. Anti-tumor effect: 3.1. Effect on cancer cells: Take the ascitic fluid of mice with liver cancer ascites that has grown well for 6-9 days, dilute it with sterile saline to 1×10(7)-2×10(7) cancer cells/ml, take this cancer cell fluid 0.5ml plus isovolum of different concentrations of crane phenol liquid, so that the final concentration of crane hydroxal is 2.5-0.08mg/ml of four concentrations, gently shake and mix well, incubate at 37 °C, and 3 hours, then take out, add 0.05% Yihong, microscopic examination, The percentage of cancer cells that were stained red was counted (dead cancer cells were reddited, and the percentage of red staining was called red infection rate), and the results of the four experiments of warm incubation for 1 hour and 3 hours were listed in the table. It was shown that 0.16mg/ml of crane hydroxal had a certain killing effect on cancer cells, and 1.25mg/ml could kill all cancer cells within 1 hour. The rate of red infection of cancer cells in the saline control group within one hour was 7.3%. 10(2)-3×10(2) cancer cells/ml, Each mouse was inoculated with 0.2 ml under the right axillary subcutaneously, each large group of animals was inoculated with a tumor source, and the next day each large group of animals was randomly divided into a drug delivery group and a normal saline control group, and began to inject 30mg/kg of crane phenol injection peritoneally every day, and the control group was injected with the same volume of normal saline, continuously administered for 12-14 days, and the weight and tumor weight of the animals were weighed the next day after the drug was stopped, and the results of repeated two batches of experiments proved that crane phenol had obvious experimental therapeutic effects on S37 and U14, and the inhibition rate of tumor growth was 47.0% (P <0.01) and 38.7% (P<0.05). It also has a certain inhibitory effect on S180 and liver cancer sarcoma, and its inhibition rates are 26.3% and 23.5% respectively (P<0.05). The dosing group animals did not die during the experiment. Anatomically observed. The difference between the size of the thymus and spleen of the animals in the drug delivery group and the control group was not obvious, and the experimental animals on the four tumor profiles increased significantly after the experiment, and the weight growth rate of the animals in the drug delivery group and the control group was similar, which showed that the dose of crane hydroxol could not see obvious toxic reactions to the experimental mice. The inhibition rate of JTC-26 is 100%. HeLA cell colony test, crane grass root contains cytotoxic components, which are soluble in methanol and ether, which can strongly inhibit HELA cell colony formation. At a concentration of 500 μg/ml, it not only does not damage normal cells, but promotes 100% growth and development of normal cells (in fact, it has a positive effect).
3.2. Effect of simultaneous local and intraperitoneal administration of tumors on solid tumors: Following the above inoculation method, mice were inoculated with S180 and 37, and 30 mg/kg of crane phenol injection was injected daily in the intraperitoneal cavity on the day after inoculation, and the control group animals were injected with the same volume of normal saline, starting on the 5th day after administration, the total amount of animal injection of crane oxalol in the dosing group was still 30mg/kg, but each animal was injected with 0.25mg in the tumor, and the remaining dose of intraperitoneal injection was administered, starting from the next day after inoculation, and administering for a total of 14 days. On day l5, he was sentenced to weigh and tumor weight, and the results showed that the inhibition rate of Zygopol to S180 was 67.7% (P<0.01). The inhibition rate of S37 was 68.1% (P<0.01). It can be seen that the efficacy of this mode of administration is more effective than that of intraperitoneal administration alone.
3.3. Effect on ascites carcinoma: liver cancer ascites carcinoma ascites fluid is extracted and diluted with sterile saline to 1×1(2)-2×10(2) cancer cells /ml, Inoculated mice (0.2 ml per mouse intraperitoneal injection) was randomly divided into the administration group and the control group on the next day, and intraperitoneal injection of crane phenol 30mg/kg once a day, continuous administration for 7 days, the control group injected with the same volume of normal saline, observed the death time of each mouse within 30 days (the undead were calculated as survival for 30 days), calculated the life extension rate results, liver cancer ascites carcinoma administration group 24 animals averaged survival of 26.2 ± 0.9 days, control group 25 animals survived an average of 17.5 ± 1.3 days. The growth prolongation rate was 49.6% (P<0.01); crane phenol had a significant life-prolonging effect on hepatic cancer ascites cancer animals.
3.4. Effect on S180: The experimental method is carried out according to the national anti-tumor drug in vivo screening protocol. The results showed that the inhibition rate of S180 in mice with intraperitoneal injection of 1.5 g/kg×10, intraperitoneal injection, and repeated experiments was 57.2% (P<0.001), 53.2% (P<0.01) and 56.2% (P<0.01), respectively; guinea pigs with transplanted sarcoma-180 were given once a day at 1000 mg/kg body weight, and the tumor suppression rate reached 37.424%; according to 100 mg/kg body weight, the tumor suppression rate reached 37.424%; Mice with intraperitoneal injection of kg body weight transplanted sarcoma-180 had an inhibition rate of 18.5% for hot water extract and 7.4% for ethanol extract, or had no antitumor effect on root aqueous extract, stem and leaf aqueous extract and alcohol extract. It is suggested that the anti-tumor component of Crane Grass is present in the root (including root buds) and is a alcohol-soluble substance. In addition, light microscopic observation showed that the tumor cells in the control group were hypertrophic and aggressive. The nucleus division phase of the drug dosing group tumor is reduced, degeneration and necrosis are severe, the cytoplasm is reticulated or vacuoleous, or even transparent, the nuclear membrane is thickened, the nuclear chromatin coagulation is coarsely granulated, and in severe cases, nuclear fragmentation and nuclear solidification. Electron microscopic observations showed that the control group of tumor cells was the same as those seen by the light microscope. Significant changes in the tumor cells of the drug dosing group expand in the nucleus, the space between the nuclear membranes, the emergence of a circular nucleoli, the phenomenon of homogenization of the nuclei. In addition, intracytoplasmic mitochondrial swelling and crest disappearance, coarse endoplasmic reticulostic vesicles and threshing, and polynucleosome disintegration have been seen.
3.5. The tumor inhibition rate of the whole grass ethanol extract on the subcutaneous type of liver cancer in mice is more than 50%.
3.6. Crane hydroxal also has inhibitory effects on mouse sarcoma-37.
4. Hemostatic effect: Clinical use of crane herb as a hemostatic agent has a history of decades, but animal experimental studies on its hemostatic effect have not reached a consensus.
4.1. Early studies have indicated that crane grass and its alcohol extracts have the effect of promoting blood clotting. Crude extracts also have the effect of promoting blood clotting, and frog legs have the effect of constricting peripheral blood vessels when perfused. After intravenous injection of Cyperus oxerarin, the bleeding time was shortened by 45%. Rabbits are injected intravenously, which can greatly shorten their blood clotting time, and the well increases the number of platelets. Experimental dog femoral artery bleeding, topical application of crane grass powder and compression, has a certain hemostasis effect.
4.2. It has been reported that the sodium salt of xianhelin 2mg/kg is given intravenously in rabbits, and the coagulation time (slide picking method), platelet count and blood coagulation decalclucization plus calcium coagulation test are reported to prove that the blood clotting time is significantly accelerated, and the platelet count is significantly increased, and it is said that xianhelin can improve the blood coagulation rate by 20%-30%, but it is also believed that xianhelin has no hemostatic effect, and it has not been confirmed that the commercially available xianhelin injection has the effect of promoting hemagglutination, and the large dose (15mg/kg) can also significantly prolong the blood clotting time The crane grass contained in the crane grass A, B, C and another phenolic resin acid has no hemagglutination effect.
5. Effect on the circulatory system: Crane grass ethanol extract to anesthetized rabbits, intravenous injections in dogs, can make blood pressure rise, and can stimulate breathing, while the alcohol extract of its water extraction part can reduce the blood pressure of rabbits. When rabbit ears and frog hind limbs are perfused with blood vessels, low concentrations cause blood vessels to shrink, and high concentrations dilate. It has also been reported that the infusion of crane grass infusion and extract perfusion of rabbit ear blood vessels has no effect at low concentrations, and the high concentration also shows a dilation reaction, and can counteract the vasoconstricting effect of epinephrine. The infusion and crane oxalin have a cardiotonic effect on the heart of the in vitro frog, which can increase the heart rate and contraction strength of frogs and toads, while the ethanol extract of the water extract has an inhibitory effect on the heart of the in vitro frog. The concentration of 1:100000-1:5000 for the ex vivo rabbit heart can increase the shrinkage amplitude, and the degree of increase is proportional to the concentration. Normal rabbits are injected intravenously 4 mg without affecting their electrocardiogram. It is shown that small doses can increase the heart contraction amplitude, medium doses can increase the heart rate and heart contraction slightly, and large doses can slow down and shrink the heart beat more.
6. Effect on smooth muscle: Ethanol extract of the water-extracted part of the body on rabbits and guinea pigs in vivo intestinal tubes, low concentration excitation, high concentration is inhibited. Crane lactone can reduce the contraction amplitude and tension of the intestines in vitro rabbits, and then stop the intestinal movements in a relaxed state. It also inhibited the peristalsis of the small intestine in place in rats.
7. Anti-inflammatory effect: Aqueous extracts and alcohols, aqueous extracts have anti-inflammatory effects on rabbit conjunctivitis caused by mustard oil or staphylococcal infection, for the former, alcohol, aqueous extract anti-inflammatory effect is better, for the latter the aqueous extract is better, in vitro tests on the inhibition of staphylococcus, but also with aqueous extract effect is stronger. Its anti-inflammatory effect lies in the astringent effect of the tannin containing the condensed type Ofan (Phlobaphene) in the crane grass.
8. Antibacterial and antiviral effect: Hot water or ethanol infusion has a certain inhibitory effect on Bacillus subtilis and Staphylococcus aureus in the test tube, and has a weak inhibitory effect on human tuberculosis bacillus. It has a killing effect on grasshoppers, and the active ingredients are soluble in ether and chloroform. The brown powder obtained by the treatment of methanol extract of crane grass has an inhibitory effect on gram-negative bacteria. The decoction of young stems and leaves of crane grass is applied topically, which also has a good effect on vaginal trichomoniasis. The aqueous extract of Agrimoniaeupatoria in Europe has an inhibitory effect on Tuberculosis bacilli in vitro tests, and is also effective for those who are resistant to streptomycin and ammoniasalicylic acid, but not to those who are resistant to isoniazid. Ethanol extract has an antiviral (ColumbiaSK virus) effect in mice.
9. Other effects: Rabbits injecting aqueous extract of 100 mg/kg have analgesic effect (rabbit pulp electrostimulation method), such as alcohol, aqueous extract 50 mg/kg is effective. In addition, it seems that the crane herb can slightly reduce blood sugar, slightly increase the resistance of red blood cells to hypotonic saline, and slightly reduce the basal metabolism of rats. In addition, winter bud powder has a laxative effect, so when clinically applied, there is no need to take another laxative.
Toxicity 1. The LD50 of the crane phenol gastric on mice was 435±88 mg/kg.
2. Taking crane phenol with cooking oil can increase toxicity, so when taking crane phenol, it cannot be used with oil. 50 mice were divided into 5 groups and each group was injected with different doses of crane phenol injection in this experiment, and the death was observed within 3 days after injection. The LD50 injected intraperitoneally in mice was obtained as 90.7±4.9 mg/kg. Each group of 10 mice with a total of 6 groups injected different doses of crane hydroxysophenone once a day, and the death situation within 12 days was observed for 10 consecutive days (administered according to the actual body weight every day), and the LD50 for 10 consecutive days of intraperitoneal injection was 67.7±4.1 mg/kg.
3. Take 210 healthy mice weighing 18-27g, male and female halves, divided into I.-IV. batches, 50-60 per batch, and each batch of animals is routinely subjected to acute toxicity experiments. Crane hydropherol is used for gastric filling. After the I.-III. rats were injected with drugs, they were respectively filled with gastric saline (control), edible soybean oil and 50% alcohol 5 ml/kg body weight. Batch IV. was mixed with 30% capacity polyethylene glycol (equivalent to 3 ml/kg) in the solution of crane phenol. After 3 days of observation, half of the lethality, standard error, and significance were determined according to the improved Karber method. The oral administration of LD50 was 599.3 mg/kg for the mice of crane herb phenol, and 540 mg/kg and 453.3 mg/kg for taking wine and edible soybean oil after administration, respectively, compared with the control group, the animals died faster and the LD50 was significantly reduced (P<0.01). Taking polyethylene glycol significantly increased LD50 (P<0.01). About 10 minutes after the mice took a large dose of crane hydroxol, the animal activity increased, and then most of the rats lay on their stomachs, closed their eyes, bowed their heads or decreased activity, and some of the animals struggled before death, and most of the animals died of muscle stiffness. Deaths occur within 1 hour of taking the drug and most die within 24 hours.
4. The toxic symptoms of crane hydroxol are mainly manifested in gastrointestinal and nervous system reactions, when a larger dose can make the domestic dog blind, pathological observation also proves the above damage, but macaque monkey oral high-dose crane phenol, in addition to the gastrointestinal reaction did not find visual impairment.
Identification (1) take 20g of this product powder, use 70% ethanol 100ml of reflux extraction for 1h, recover at least the amount of ethanol, for the following tests: (1) take 2ml of supply test solution, add 5% vanillin concentrated sulfuric acid solution 2ml, the interface is reddish-brown ring. (Check the phenols) (2) Take 2 ml of the test solution, add 1 ml of the 3 ferric chloride test solution, and it will be dirty green. (Check tannin) (3) Take 2 ml of the test solution, add 2 ml of 5% gelatin solution, and produce a white precipitate. (Check tannin) (4) Take 2ml of test solution, add a little magnesium powder and 3-5 drops of concentrated hydrochloric acid, showing cherry red. (Check flavonoids) (2) Thin layer chromatography Take 10g of this product powder, use 50ml of petroleum ether (boiling range 60-90 °C) reflux extraction for 90min, filtered. The filtrate evaporates the petroleum ether and dissolves it with 5ml of chloroform as a test solution. Take a little of the hydroxysophen and dissolve it with chloroform as a control solution. Take the sample and control solution, dotted on the same silica gel G (Qingdao) thin layer plate, with n-hexane-ethyl acetate-glacial acetic acid (20:25:0.7) upward unfolding, the spread distance is about 15cm. Remove to dry. After being sprayed with concentrated sulfuric acid and heated, spots of the same color appear in the test chromatography corresponding to the control chromatography.
Harvested when the foliage is flourishing before flowering.
The sexual taste is bitter, astringent, and the sex is flat.
Attribution to the lungs; liver; spleen meridians
Functional indications for astringent hemostasis, diarrhea, insecticide. Main hemoptysis; vomiting blood; blood in urine; blood in the stool; red diarrhea; under the collapse belt; exertion and detachment; carbuncle; bruises; traumatic bleeding
Dosage for internal use: decoction, 10-15g, large doses can be used 30g; or into powder. For external use: mashing; or applying boiling paste.
Note that those who do not bleed non-stop do not use it.
Each family discussed 1. "Lu Wei Yan Materia Medica": Leaf: Cure sore ringworm.
2. "Yunnan Materia Medica": cure women's menstruation or before or after, red and white belt, cold and abdominal pain, long-term red and white blood diarrhea.
3. "Raw Herbal Preparation": rationalize bruises, stop bleeding, and scatter sores and poisons.
4. "Hundred Grass Mirror": blood circulation under the qi, rationalization of hundreds of diseases, scattered and full; falling and vomiting blood, blood collapse, diarrhea, intestinal wind under the blood.
5. "Botanical Names and Facts": Cure wind phlegm and low back pain.
6. "Counterfeit Medicine Article Identification": Cure fistula.
7. "Modern Practical Chinese Medicine": It is a strong astringent hemostatic agent, and has a cardiotonic effect. It is used in lung disease hemoptysis, intestinal bleeding, gastric ulcer bleeding, uterine bleeding, dental bleeding, hemorrhoidal hemorrhoids, liver abscess and other symptoms.
Excerpt from "Chinese Materia Medica"
