Recently, the pesticide chemistry and application innovation team of the Institute of Plant Protection of the Chinese Academy of Agricultural Sciences prepared a new type of microplate label antibody complex, which greatly improved the sensitivity and antibody stability of the rapid detection of mycotoxin pollutants. The results of the research were published online in the journal of hazardous materials.
According to researcher Zheng Yongquan, the traditional enzyme label antibody is usually prepared by enzyme and antibody through covalent binding, due to the cumbersome covalent binding process, the catalytic activity of the enzyme and the recognition ability of the antibody are unstable, often resulting in a decrease in the detection sensitivity of the immune system. In addition, the stability of the microplate label antibody is highly susceptible to the influence of the external environment. Therefore, in the practical application of immunoassays, the development of a method to prepare a simple while maintaining the efficient activity of microplate label antibodies is a technical difficulty in the relevant field.
Schematic diagram of the preparation of novel microplate label antibodies (a) and their application in the high sensitivity and rapid detection of mycotoxins (b).
Therefore, the study proposes a novel strategy for preparing microplate label antibodies, using the mof material to design a magical "coat" for enzymes and antibodies. It is capable of tight in situ fusion of horseradish peroxidase (hrp) and sheep anti-murine monoclonal antibodies (ab) to form microplate label antibodies (hrp/ab@mof). After wearing this "coat", the microplate label antibody is protected from the influence of the external temperature, organic solvents and other environments, and the stability, catalytic activity and recognition ability of the fusion microbizymal antibody are greatly improved compared with the traditional free horseradish peroxidase and sheep anti-mouse monoclonal antibodies. This strategy was successfully used for the highly sensitive and rapid determination of the mycotoxin zearalenone, which was 126 times more sensitive than that of traditional immunoassay methods.
It is understood that the research has been funded by projects such as the National Key Research and Development Program.
Source: Guangming Network