Giardia and Cryptosporidium (bisporidium) rapid detection system tender parameters

Giardia and Cryptosporidium (bisporidium) rapid detection system tender parameters

First, the use of equipment

Used to detect cryptosporidium and Giardia in water.

Second, the configuration of technical indicators

1. Rapid pressure filtration and washing device

1.1 System composition: a set of reusable filter sleeves and connecting accessories, peristaltic pump, automatic rapid pressure elution device and centrifuge.

1.2 Method principle: After sampling and washing by rapid method, immunomagnetic separation and fluorescence analysis and detection are carried out.

1.3 Compliance with:

★1.3.1 GB/T 5750-2006 "Drinking Water Standard Inspection Method" one of the three testing methods.

1.4. Technical indicators:

1.4.1 Average recovery rate: greater than 35%.

1.4.2 Filtration/elution recovery rate: 60-90%, stability greater than 70%.

1.4.3 Relative standard deviation: less than 20%.

1.4.4 Water sample treatment capacity: tap water ≥ 100L, water source water ≥ 20L.

1.4.5 Maximum turbidity of treatable water samples: >50NTU.

★1.4.6 Sampling maximum flow rate: ≥4 l/min.

★1.4.7 Pressure elution device: fully automatic and rapid backwashing of compressed air and buffer is applied to the collected samples, the number of elutions of each sample is greater than 8 times, and the total elution time of each sample is less than 2 minutes.

1.4.8 Minimum input pressure: ≥4.5 bar (65 psi) Maximum input pressure: ≥ 10.3 bar (150 psi).

1.4.9 Operating pressure: 5-8 bar (72.5-116 psi).

1.4.10 Output power: 100-240VAC, 47-63Hz; output 4.2-5.0A, 24VDC.

2. Basic parameters of peristaltic pump

2.1, pump head ≥ 7.6 cm.

2.2, volume ≤ 34.2x25.2x23 (LxWxH) (cm).

2.3, power ≤ 0.25 kw.

2.4, flow rate 0.6-8 L/min.

3. Technical parameters of centrifuge

3.1, system composition: floor-standing centrifuge, 500mlx4 Erlenmeyer-type centrifuge bottle.

3.2. The centrifuge is used for the collection and centrifugation of washed samples in the rapid detection system of Cryptosporidium and Giardia, and the Erlenmeyer-shaped centrifugal bottle is required to be used in conjunction with the fixed recovery position of the elution device, and the entire centrifugation process meets the experimental requirements of the detection of two insects.

3.3, the maximum capacity of suspension horizontal rotor can reach 2000ml (500mlx4), the maximum speed is 9000rpm, the maximum centrifugal force: ≥9600g.

3.4. Technical parameters:

(1) Speed control microprocessor.

(2) Temperature range -9 ~ 35 °C.

4. Research-grade upright fluorescence microscope

4.1, microscope host:

4.1.1, research-grade upright microscope, can be used for brightfield, fluorescence, differential interference difference observation.

4.1.2, optical system: infinity correction optical system, the focal length must be the international standard of 45mm.

4.1.3, focus: fine-tuning accuracy ≤ 1 micron.

4.1.4, observation lens tube: wide field of view trinocular lens tube, inclination angle of 30 °, three-stage optical path 100%:0 0:100% 20%:80%。

4.1.5, lighting device: built-in transmitted light Kohler illuminator, with light intensity preset button, the second generation of light intensity management button, LED power ≥ 100W. 100W or higher power reflected fluorescent illumination device, carry out fluorescent U and B excitation observation.

4.1.6 Objective:

20X/0.5，WD 1.6 (spring)

40X/0.75，WD 0.51

100X/1.3，WD 0.2 (spring，oil)

4.1.7. Stage: High-wear-resistant ceramic overlay stage with low right hand coaxial drive knob.

4.1.8, eyepiece: 10X wide field of view eyepiece.

4.1.9, objective converter: six-hole objective converter.

4.1.10, condenser: multi-functional eight-hole condenser.

4.1.11, differential interference systems: polarization mirror, polarization mirror, Nomaski prism.

4.2. Microscopic image analysis system:

4.2.1, with cooling function high resolution microscopy dedicated digital camera.

4.2.2, chip specifications: 1/1.2 inch, 2.35M color CMOS.

4.2.3, the number of fields of view collected ≥21.

4.2.4、 Image speed: ≥60 frames/sec @ 1920 x 1200.

4.2.5、 Image transmission speed: ≥60 frames/sec @ 1920 x 1200.

4.2.6, maximum image resolution: ≥ 20.7 million, 5760 X 3600 (pixel movement).

4.2.7, metering mode: manual, auto, super fluorescence auto (SFL).

4.2.8, equipped with the same brand of image acquisition and processing analysis software, and a set of workstations.

remark:

The parameters of the above numbering ★ are important parameters of this tender project.

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