Recently, the Nitzan Shabek Research Group of the Department of Plant Biology at the University of California, Davis, published a research paper entitled A conformational switch in the SCF-D3/MAX2 ubiquitin ligase facilitates strigolactone signalling online. The study used structural biology and CRISPR to explore the new functions of MAX2 E3-ligase in the one-legged gold signaling pathway and proposed a new regulatory model.
One-legged golden lactones (SLs) are a class of plant hormones that regulate many processes of growth and development. Signal transduction of SL involves interactions between F-box E3 ubiquitin ligase D3/MAX2 and DWARF14(D14)α/β-hydrolase and degrades targets of D53/SMXL6/7/8 transcriptional inhibitory factors (SMXLs) in an SL-dependent manner. D3/MAX2 has been shown to have multiple conformational states (Nature, 2018 by Shabek), whose C-terminal spiral (CTH) undergoes dynamic changes from closed to open, and regulates the binding of D14 and senses the one-legged golden lactone. Although multiple interaction patterns of D3-D14 have been found in vitro, the residues that regulate the conformational conversion of D3/MAX2 CTH when targeting D53/SMXL and the subsequent effects on SL signals remain unclear. This study uses crystallography to design protein conformation conversion mutants, elucidate the functional dynamics of ASK1-D3/MAX2 in SL signaling, and further use CRISPR technology to verify in Arabidopsis thaliana, and then elucidate the functional dynamics of ASK1-D3/MAX2 in SL signaling and propose a new regulatory model.
Fig. 1 | Structural and functional characterization of dislodged ASK1–D3-CTH
Fig. 3 | Functional characterization of D-pocket perturbed ASK1–D3 mutant in systemic SL signalling.
Fig. 6 | CRISPR–Cas9-guided mutagenesis of CTH of MAX2 results in SL-signalling deficiency phenotypes
Fig. 7 | An updated model for SL signalling.
The research group postdoc Lior Tal was the first author, Nitzan Shabek was the corresponding author, and postdoc Malathy Palayam and research assistant Alec Young were also involved in the work.
original:
https://www.nature.com/articles/s41477-022-01145-7
Scientific research services (cooperative investment)
Patent Application | Rapid Detection of Transgenic Plants| Tissue Culture| Yeast Bi-Hybrid | Genetics Technical Services, SNP Typing, High Homologous Segment SNP Typing, Target Interval Resequencing, Methylation Sequencing | Plant antibodies |
The forefront of plant science, focusing on the frontier progress of plant science, information, the release of recruitment information and method software sharing. For submission and recruitment, please reply to the "submission" in the background, all of which are free; for business cooperation, please contact WeChat ID: zwkxqy;