You see, Arabidopsis thaliana in the literature
If ps is not getting started, the channel keying looks complicated. Follow my tutorial and click the mouse to get it done.
The software website is searchable for imageJ Fiji editions
Software Introduction:
ImageJ is a public image processing software developed on Java, developed by the National Institutes of Health, based on Java features, it can naturally be used across platforms (Windows, Linux, Mac OS).
With memory permitting, ImageJ can open as many images as it wants for processing. In addition to basic image operations such as scaling, rotating, distorting, smoothing, ImageJ can also perform image area and pixel statistics, spacing, angle calculations, create histograms and profiles, and when there is also cell counting (not limited to cells), WB protein quantification, and object area length calculations under a reference ruler.
【Keying operation steps are as follows】
1、File → Open
2、Image→Type→Lab Stack
3. Select the second mouse wheel
4. Image → Duplicate (Ctrl+Shift+D) to copy the layer
5、Image→Adjust→Brightness/Contrast（Ctrl+Shift+C）
（Ctrl+Shift+C）
6、Image→Type→8 bit
7、Image→Adjust→Threshold（Ctrl+Shift+T）→ apply
8、Process→Filters→Median
9. Use the magic wand tool to tap on the black area
10、Edit→Selection→Create Selection
11. File →Open original image
12、Edit→Selection→Restore Selection
13、Edit→Clear Outside
All of the above steps are to get the outline of Arabidopsis thaliana and then delete the background other than the selection.
At last
Peng Duanshu of the Qing Dynasty said in "A Show of Sons and Nephews for Learning", "Is it difficult or easy to do things in the world? For this, it is easy to be difficult; if it is not, it is also difficult to be easy. Is it difficult to learn human beings? If you learn, it is easy to learn, and if you don't learn, it is easy to learn. ”
Good luck to everyone, SCI sent to the soft hand ~